TY - JOUR
T1 - Restricted heterogeneity in T-cell antigen receptor V(β) gene usage in the lymph nodes and arthritic joints of mice
AU - Haqqi, T. M.
AU - Anderson, G. D.
AU - Banerjee, S.
AU - David, C. S.
PY - 1992
Y1 - 1992
N2 - We have used PCR to study the expression of T-cell antigen receptor β RNA containing particular variable region (V) elements from transcripts directly in the cells isolated from joints and lymph nodes of B10.Q mice (H-2(q)) immunized with chicken type II collagen. Our data show that the T cells present in arthritic joints expressed only a few V(β) transcripts-V(β)2, - 6, -7, -8.2, -9, -10, and -15. V(β)6 and -8.2 were expressed predominantly (six out of seven animals) while others were expressed at a relatively low level in different animals. In lymph node cells, transcripts for V(β)6, - 8.2, and -9 were detected in four out of seven animals. The data indicate that in collagen-induced arthritis there is a restrictive usage of TCR V(β) elements and that V(β)6 and -8.2 are probably used preferentially.
AB - We have used PCR to study the expression of T-cell antigen receptor β RNA containing particular variable region (V) elements from transcripts directly in the cells isolated from joints and lymph nodes of B10.Q mice (H-2(q)) immunized with chicken type II collagen. Our data show that the T cells present in arthritic joints expressed only a few V(β) transcripts-V(β)2, - 6, -7, -8.2, -9, -10, and -15. V(β)6 and -8.2 were expressed predominantly (six out of seven animals) while others were expressed at a relatively low level in different animals. In lymph node cells, transcripts for V(β)6, - 8.2, and -9 were detected in four out of seven animals. The data indicate that in collagen-induced arthritis there is a restrictive usage of TCR V(β) elements and that V(β)6 and -8.2 are probably used preferentially.
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U2 - 10.1073/pnas.89.4.1253
DO - 10.1073/pnas.89.4.1253
M3 - Article
C2 - 1311091
AN - SCOPUS:0026570859
VL - 89
SP - 1253
EP - 1255
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 4
ER -