We have used PCR to study the expression of T-cell antigen receptor β RNA containing particular variable region (V) elements from transcripts directly in the cells isolated from joints and lymph nodes of B10.Q mice (H-2(q)) immunized with chicken type II collagen. Our data show that the T cells present in arthritic joints expressed only a few V(β) transcripts-V(β)2, - 6, -7, -8.2, -9, -10, and -15. V(β)6 and -8.2 were expressed predominantly (six out of seven animals) while others were expressed at a relatively low level in different animals. In lymph node cells, transcripts for V(β)6, - 8.2, and -9 were detected in four out of seven animals. The data indicate that in collagen-induced arthritis there is a restrictive usage of TCR V(β) elements and that V(β)6 and -8.2 are probably used preferentially.
|Original language||English (US)|
|Number of pages||3|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1992|
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