Restoration ofmiR-101 suppresses lung tumorigenesis through inhibition of DNMT3a-dependent DNA methylation

F. Yan, N. Shen, J. Pang, D. Xie, B. Deng, Julian R Molina, Ping Yang, S. Liu

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The deregulation of miR-101 and DNMT3a has been implicated in the pathogenesis of multiple tumor types, but whether and how miR-101 silencing and DNMT3a overexpression contribute to lung tumorigenesis remain elusive. Here we show that miR-101 downregulation associates with DNMT3a overexpression in lung cancer cell lines and patient tissues. Ectopic miR-101 expression remarkably abrogated the DNMT3a 30-UTR luciferase activity corresponding to the miR-101 binding site and caused an attenuated expression of endogenous DNMT3a, which led to a reduction of global DNA methylation and the re-expression of tumor suppressor CDH1 via its promoter DNA hypomethylation. Functionally, restoration of miR-101 expression suppressed lung cancer cell clonability and migration, which recapitulated the DNMT3a knockdown effects. Interestingly, miR-101 synergized with decitabine to downregulate DNMT3a and to reduce DNA methylation. Importantly, ectopic miR-101 expression was sufficient to trigger in vivo lung tumor regression and the blockage of metastasis. Consistent with these phenotypes, examination of xenograft tumors disclosed an increase of miR-101, a decrease of DNMT3a and the subsequent DNA demethylation. These findings support that the loss or suppression of miR-101 function accelerates lung tumorigenesis through DNMT3a-dependent DNA methylation, and suggest that miR-101-DNMT3a axis may have therapeutic value in treating refractory lung cancer.

Original languageEnglish (US)
Article numbere1413
JournalCell Death and Disease
Volume5
Issue number9
DOIs
StatePublished - Jan 1 2014

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DNA Methylation
Carcinogenesis
Lung Neoplasms
Lung
decitabine
Neoplasms
Down-Regulation
Untranslated Regions
DNA
Luciferases
Heterografts
Cell Movement
Binding Sites
Neoplasm Metastasis
Phenotype
Cell Line
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience

Cite this

Restoration ofmiR-101 suppresses lung tumorigenesis through inhibition of DNMT3a-dependent DNA methylation. / Yan, F.; Shen, N.; Pang, J.; Xie, D.; Deng, B.; Molina, Julian R; Yang, Ping; Liu, S.

In: Cell Death and Disease, Vol. 5, No. 9, e1413, 01.01.2014.

Research output: Contribution to journalArticle

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AU - Molina, Julian R

AU - Yang, Ping

AU - Liu, S.

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