TY - JOUR
T1 - Restoration of density of interstitial cells of cajal in the jejunum of diabetic rats after quercetin supplementation
AU - Vieira-Frez, Flávia Cristina
AU - Martins-Colombo-Perles, Juliana Vanessa
AU - Robert-Linden, David
AU - Gibbons, Simon James
AU - Amilcar-Martins, Heber
AU - Almeida-Brito-Romualdo, Débora
AU - De-Souza, Sara Raquel
AU - Daion-Piovezana-Bossolani, Gleison
AU - Zanoni, Jacqueline Nelisis
N1 - Publisher Copyright:
© 2017. SEPD y ARÁN EDICIONES, S.L.
PY - 2017
Y1 - 2017
N2 - Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICCMY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.
AB - Background: Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy. Aim: To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats. Methods: Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed. Results: The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICCMY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05). Conclusions: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.
KW - Antioxidants
KW - Diabetes mellitus
KW - Interstitial cells of Cajal
KW - Quercetin
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UR - http://www.scopus.com/inward/citedby.url?scp=85025072016&partnerID=8YFLogxK
U2 - 10.17235/reed.2016.4338/2016
DO - 10.17235/reed.2016.4338/2016
M3 - Article
C2 - 28004965
AN - SCOPUS:85025072016
SN - 1130-0108
VL - 109
SP - 190
EP - 195
JO - Revista Espanola de Enfermedades Digestivas
JF - Revista Espanola de Enfermedades Digestivas
IS - 3
ER -