Restoration of Ca2+-inhibited oxidative phosphorylation in cardiac mitochondria by mitochondrial Ca2+ unloading

E. L. Holmuhamedov, C. Ozcan, A. Jahangir, A. Terzic

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Mitochondria, the major source of cellular ATP, display high vulnerability to metabolic stress, in particular to excessive Ca2+ loading. Here, we show that Ca2+-inhibited mitochondrial ATP generation could be restored through stimulated Ca2+ discharge from mitochondrial matrix. This was demonstrated using a Ca2+ ionophore or through Na+/Ca2+ exchange-mediated decrease of mitochondrial Ca2+ load. Furthermore, diazoxide, a mitochondrial potassium channel opener, which maintained mitochondrial Ca2+ homeostasis, also restored Ca2+-inhibited ATP synthesis and preserved the structural integrity of Ca2+-challenged mitochondria. Thus, under conditions of excessive mitochondrial Ca2+ overload targeting mitochondrial Ca2+ transport pathways restores oxidative phosphorylation required for vital cellular processes. This study, therefore, identifies an effective strategy capable to rescue Ca2+-disrupted mitochondrial energetics.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume220
Issue number1-2
DOIs
StatePublished - Jun 21 2001

Keywords

  • ATP
  • Bioenergetics
  • Ca overload
  • Cardioprotection
  • Diazoxide
  • Mitochondria
  • Potassium channel opener

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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