TY - JOUR
T1 - Responses of endoscopy patients in Ladakh, India, to Helicobacter pylori whole-cell and CagA antigens
AU - Romero-Gallo, Judith
AU - Pérez-Pérez, Guillermo I.
AU - Novick, Richard P.
AU - Kamath, Patrick
AU - Norbu, Tsering
AU - Blaser, Martin J.
PY - 2002/11
Y1 - 2002/11
N2 - Although Helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom H. pylori was cultured have not been studied. We report on studies of 189 persons in the Ladakh region of India in whom serum immunoglobulin G responses to H. pylori whole-cell and CagA antigens were measured. H. pylori was isolated from 68 of these patients. An H. pylori whole-cell antigen derived from Ladakhi strains out-performed a similar antigen from U.S. strains, as determined by antigenspecific enzyme-linked immunosorbent assays. In total, 95% of the population was seropositive, including individuals responding only to the CagA antigen. Correlation with culture results showed that these were true positives and, therefore, that the H. pylori whole-cell serology was falsely negative in some cases. In addition to establishing a collection of H. pylori isolates from a remote area in the world, we show that use of H. pylori whole-cell and CagA serology together increases the sensitivity for the detection of colonization.
AB - Although Helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom H. pylori was cultured have not been studied. We report on studies of 189 persons in the Ladakh region of India in whom serum immunoglobulin G responses to H. pylori whole-cell and CagA antigens were measured. H. pylori was isolated from 68 of these patients. An H. pylori whole-cell antigen derived from Ladakhi strains out-performed a similar antigen from U.S. strains, as determined by antigenspecific enzyme-linked immunosorbent assays. In total, 95% of the population was seropositive, including individuals responding only to the CagA antigen. Correlation with culture results showed that these were true positives and, therefore, that the H. pylori whole-cell serology was falsely negative in some cases. In addition to establishing a collection of H. pylori isolates from a remote area in the world, we show that use of H. pylori whole-cell and CagA serology together increases the sensitivity for the detection of colonization.
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U2 - 10.1128/CDLI.9.6.1313-1317.2002
DO - 10.1128/CDLI.9.6.1313-1317.2002
M3 - Article
C2 - 12414766
AN - SCOPUS:0036842765
SN - 1071-412X
VL - 9
SP - 1313
EP - 1317
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 6
ER -