Responses of endoscopy patients in Ladakh, India, to Helicobacter pylori whole-cell and CagA antigens

Judith Romero-Gallo, Guillermo I. Pérez-Pérez, Richard P. Novick, Patrick Kamath, Tsering Norbu, Martin J. Blaser

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48 Scopus citations

Abstract

Although Helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom H. pylori was cultured have not been studied. We report on studies of 189 persons in the Ladakh region of India in whom serum immunoglobulin G responses to H. pylori whole-cell and CagA antigens were measured. H. pylori was isolated from 68 of these patients. An H. pylori whole-cell antigen derived from Ladakhi strains out-performed a similar antigen from U.S. strains, as determined by antigenspecific enzyme-linked immunosorbent assays. In total, 95% of the population was seropositive, including individuals responding only to the CagA antigen. Correlation with culture results showed that these were true positives and, therefore, that the H. pylori whole-cell serology was falsely negative in some cases. In addition to establishing a collection of H. pylori isolates from a remote area in the world, we show that use of H. pylori whole-cell and CagA serology together increases the sensitivity for the detection of colonization.

Original languageEnglish (US)
Pages (from-to)1313-1317
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume9
Issue number6
DOIs
StatePublished - Nov 1 2002

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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