TY - JOUR
T1 - Response of human ciliated respiratory cells to a mixture of menthol, eucalyptus oil and pine needle oil
AU - Riechelmann, Herbert
AU - Brommer, Christian
AU - Hinni, Mike
AU - Martin, Cornel
PY - 1997
Y1 - 1997
N2 - Nasal respiratory cells were harvested from 45 healthy individuals using a microcurette technique. Following harvest, cells were placed on microporous polycarbonate membranes and exposed for 30 min using a gas/liquid interface technique to a mixture of menthol, eucalyptus oil and pine needle oil (MEP), eucalyptus oil alone (ECO), and pine needle oil alone (PNO) at concentrations ranging between 0.2 and 11 g/m3. Ambient air served as control. Ciliary beat frequency (CBF) was assessed before and after exposure using video-interference-contrast microscopy. Control exposure to air (n = 10) did not alter CBF (7.1 ± 0.9 Hz before and 7.0 ± 0.9 Hz after exposure, -1.3% decrease), whereas a dose dependent decrease of CBF following exposure to MEP (max. decrease -22.6% at a concentration of 10 g/m3, p < 0.01), ECO (max. decrease -32.5% at a concentration of 7.5 g/m3, p < 0.01) and PNO (max. decrease -56.1% at a concentration of 9.4 g/m3, p < 0.01) was observed. The data show that essential oils in high concentrations can reduce in-vitro ciliary activity of human respiratory cells not protected by a mucus layer. These effects have to be verified by in vivo investigations.
AB - Nasal respiratory cells were harvested from 45 healthy individuals using a microcurette technique. Following harvest, cells were placed on microporous polycarbonate membranes and exposed for 30 min using a gas/liquid interface technique to a mixture of menthol, eucalyptus oil and pine needle oil (MEP), eucalyptus oil alone (ECO), and pine needle oil alone (PNO) at concentrations ranging between 0.2 and 11 g/m3. Ambient air served as control. Ciliary beat frequency (CBF) was assessed before and after exposure using video-interference-contrast microscopy. Control exposure to air (n = 10) did not alter CBF (7.1 ± 0.9 Hz before and 7.0 ± 0.9 Hz after exposure, -1.3% decrease), whereas a dose dependent decrease of CBF following exposure to MEP (max. decrease -22.6% at a concentration of 10 g/m3, p < 0.01), ECO (max. decrease -32.5% at a concentration of 7.5 g/m3, p < 0.01) and PNO (max. decrease -56.1% at a concentration of 9.4 g/m3, p < 0.01) was observed. The data show that essential oils in high concentrations can reduce in-vitro ciliary activity of human respiratory cells not protected by a mucus layer. These effects have to be verified by in vivo investigations.
KW - Eucalyptus oil
KW - Menthol
KW - Mucociliary clearance
KW - Oils, volatile
KW - Pine needle oil
KW - Respiratory mucosa
UR - http://www.scopus.com/inward/record.url?scp=0030853683&partnerID=8YFLogxK
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M3 - Article
C2 - 9342418
AN - SCOPUS:0030853683
SN - 0004-4172
VL - 47
SP - 1035
EP - 1039
JO - Arzneimittel-Forschung/Drug Research
JF - Arzneimittel-Forschung/Drug Research
IS - 9
ER -