Response of GAP-43 and p75 in human neuromas over time after traumatic injury

Holly S. Gilmer-Hill, Roger Beuerman, Qiangwei Ma, Jing Jiang, Robert L. Tiel, David G. Kline, Corey Raffel, Robert J. Spinner

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: GAP-43 and p75 are proteins that promote growth cone and neurite formation, elongation, and arborization in regenerating nerve axons. The objectives of this study were to determine whether GAP-43 and the low-affinity nerve growth factor receptor p75 are elevated in traumatic neuromas and whether there is a correlation between the relative amount of GAP-43 or p75 and demographic characteristics such as time elapsed between injury and repair. METHODS: Traumatic neuromas from 21 randomly selected patients were studied, and the charts were reviewed. Specimens were collected at the time of nerve resection and grafting. Immunohistochemical analysis was performed on each sample and normal human nerve with antibodies to GAP-43 and p75. Western blot and computerized gel analyses were performed. RESULTS: All neuroma specimens harvested within 13 months of injury exhibited markedly elevated GAP-43 levels compared with normal nerve. Specimens harvested at 14 months or more after injury showed precipitously lower GAP-43 levels, similar to or less than those of normal nerve. The correlation between the amount of intraaxonal GAP-43 and postinjury time interval was statistically significant, P = 0.0038. High GAP-43 levels were also correlated with transection injury, high postoperative sensory grade, and pain. p75 levels were elevated, without consistent variation in our population. CONCLUSION: These preliminary data suggest that the expression of intra-axonal GAP-43 may vary over time after injury, remaining elevated for approximately the first year, then decreasing abruptly to normal or subnormal levels. These results correlate with clinical experience, indicating that peripheral nerves should be repaired relatively early if repair is indicated.

Original languageEnglish (US)
Pages (from-to)1229-1237
Number of pages9
JournalNeurosurgery
Volume51
Issue number5
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

GAP-43 Protein
Neuroma
Wounds and Injuries
Nerve Growth Factor Receptor
Growth Cones
Neurites
Peripheral Nerves
Axons
Western Blotting
Gels
Demography
Pain

Keywords

  • GAP-43
  • Growth-associated protein
  • Low-affinity nerve growth factor receptor
  • Nerve injury
  • Neuroma
  • P75

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Gilmer-Hill, H. S., Beuerman, R., Ma, Q., Jiang, J., Tiel, R. L., Kline, D. G., ... Spinner, R. J. (2002). Response of GAP-43 and p75 in human neuromas over time after traumatic injury. Neurosurgery, 51(5), 1229-1237. https://doi.org/10.1097/00006123-200211000-00019

Response of GAP-43 and p75 in human neuromas over time after traumatic injury. / Gilmer-Hill, Holly S.; Beuerman, Roger; Ma, Qiangwei; Jiang, Jing; Tiel, Robert L.; Kline, David G.; Raffel, Corey; Spinner, Robert J.

In: Neurosurgery, Vol. 51, No. 5, 01.11.2002, p. 1229-1237.

Research output: Contribution to journalArticle

Gilmer-Hill, HS, Beuerman, R, Ma, Q, Jiang, J, Tiel, RL, Kline, DG, Raffel, C & Spinner, RJ 2002, 'Response of GAP-43 and p75 in human neuromas over time after traumatic injury', Neurosurgery, vol. 51, no. 5, pp. 1229-1237. https://doi.org/10.1097/00006123-200211000-00019
Gilmer-Hill HS, Beuerman R, Ma Q, Jiang J, Tiel RL, Kline DG et al. Response of GAP-43 and p75 in human neuromas over time after traumatic injury. Neurosurgery. 2002 Nov 1;51(5):1229-1237. https://doi.org/10.1097/00006123-200211000-00019
Gilmer-Hill, Holly S. ; Beuerman, Roger ; Ma, Qiangwei ; Jiang, Jing ; Tiel, Robert L. ; Kline, David G. ; Raffel, Corey ; Spinner, Robert J. / Response of GAP-43 and p75 in human neuromas over time after traumatic injury. In: Neurosurgery. 2002 ; Vol. 51, No. 5. pp. 1229-1237.
@article{4ea50e0cc08e46d79a0a57fa5099d462,
title = "Response of GAP-43 and p75 in human neuromas over time after traumatic injury",
abstract = "OBJECTIVE: GAP-43 and p75 are proteins that promote growth cone and neurite formation, elongation, and arborization in regenerating nerve axons. The objectives of this study were to determine whether GAP-43 and the low-affinity nerve growth factor receptor p75 are elevated in traumatic neuromas and whether there is a correlation between the relative amount of GAP-43 or p75 and demographic characteristics such as time elapsed between injury and repair. METHODS: Traumatic neuromas from 21 randomly selected patients were studied, and the charts were reviewed. Specimens were collected at the time of nerve resection and grafting. Immunohistochemical analysis was performed on each sample and normal human nerve with antibodies to GAP-43 and p75. Western blot and computerized gel analyses were performed. RESULTS: All neuroma specimens harvested within 13 months of injury exhibited markedly elevated GAP-43 levels compared with normal nerve. Specimens harvested at 14 months or more after injury showed precipitously lower GAP-43 levels, similar to or less than those of normal nerve. The correlation between the amount of intraaxonal GAP-43 and postinjury time interval was statistically significant, P = 0.0038. High GAP-43 levels were also correlated with transection injury, high postoperative sensory grade, and pain. p75 levels were elevated, without consistent variation in our population. CONCLUSION: These preliminary data suggest that the expression of intra-axonal GAP-43 may vary over time after injury, remaining elevated for approximately the first year, then decreasing abruptly to normal or subnormal levels. These results correlate with clinical experience, indicating that peripheral nerves should be repaired relatively early if repair is indicated.",
keywords = "GAP-43, Growth-associated protein, Low-affinity nerve growth factor receptor, Nerve injury, Neuroma, P75",
author = "Gilmer-Hill, {Holly S.} and Roger Beuerman and Qiangwei Ma and Jing Jiang and Tiel, {Robert L.} and Kline, {David G.} and Corey Raffel and Spinner, {Robert J.}",
year = "2002",
month = "11",
day = "1",
doi = "10.1097/00006123-200211000-00019",
language = "English (US)",
volume = "51",
pages = "1229--1237",
journal = "Neurosurgery",
issn = "0148-396X",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Response of GAP-43 and p75 in human neuromas over time after traumatic injury

AU - Gilmer-Hill, Holly S.

AU - Beuerman, Roger

AU - Ma, Qiangwei

AU - Jiang, Jing

AU - Tiel, Robert L.

AU - Kline, David G.

AU - Raffel, Corey

AU - Spinner, Robert J.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - OBJECTIVE: GAP-43 and p75 are proteins that promote growth cone and neurite formation, elongation, and arborization in regenerating nerve axons. The objectives of this study were to determine whether GAP-43 and the low-affinity nerve growth factor receptor p75 are elevated in traumatic neuromas and whether there is a correlation between the relative amount of GAP-43 or p75 and demographic characteristics such as time elapsed between injury and repair. METHODS: Traumatic neuromas from 21 randomly selected patients were studied, and the charts were reviewed. Specimens were collected at the time of nerve resection and grafting. Immunohistochemical analysis was performed on each sample and normal human nerve with antibodies to GAP-43 and p75. Western blot and computerized gel analyses were performed. RESULTS: All neuroma specimens harvested within 13 months of injury exhibited markedly elevated GAP-43 levels compared with normal nerve. Specimens harvested at 14 months or more after injury showed precipitously lower GAP-43 levels, similar to or less than those of normal nerve. The correlation between the amount of intraaxonal GAP-43 and postinjury time interval was statistically significant, P = 0.0038. High GAP-43 levels were also correlated with transection injury, high postoperative sensory grade, and pain. p75 levels were elevated, without consistent variation in our population. CONCLUSION: These preliminary data suggest that the expression of intra-axonal GAP-43 may vary over time after injury, remaining elevated for approximately the first year, then decreasing abruptly to normal or subnormal levels. These results correlate with clinical experience, indicating that peripheral nerves should be repaired relatively early if repair is indicated.

AB - OBJECTIVE: GAP-43 and p75 are proteins that promote growth cone and neurite formation, elongation, and arborization in regenerating nerve axons. The objectives of this study were to determine whether GAP-43 and the low-affinity nerve growth factor receptor p75 are elevated in traumatic neuromas and whether there is a correlation between the relative amount of GAP-43 or p75 and demographic characteristics such as time elapsed between injury and repair. METHODS: Traumatic neuromas from 21 randomly selected patients were studied, and the charts were reviewed. Specimens were collected at the time of nerve resection and grafting. Immunohistochemical analysis was performed on each sample and normal human nerve with antibodies to GAP-43 and p75. Western blot and computerized gel analyses were performed. RESULTS: All neuroma specimens harvested within 13 months of injury exhibited markedly elevated GAP-43 levels compared with normal nerve. Specimens harvested at 14 months or more after injury showed precipitously lower GAP-43 levels, similar to or less than those of normal nerve. The correlation between the amount of intraaxonal GAP-43 and postinjury time interval was statistically significant, P = 0.0038. High GAP-43 levels were also correlated with transection injury, high postoperative sensory grade, and pain. p75 levels were elevated, without consistent variation in our population. CONCLUSION: These preliminary data suggest that the expression of intra-axonal GAP-43 may vary over time after injury, remaining elevated for approximately the first year, then decreasing abruptly to normal or subnormal levels. These results correlate with clinical experience, indicating that peripheral nerves should be repaired relatively early if repair is indicated.

KW - GAP-43

KW - Growth-associated protein

KW - Low-affinity nerve growth factor receptor

KW - Nerve injury

KW - Neuroma

KW - P75

UR - http://www.scopus.com/inward/record.url?scp=0036871435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036871435&partnerID=8YFLogxK

U2 - 10.1097/00006123-200211000-00019

DO - 10.1097/00006123-200211000-00019

M3 - Article

C2 - 12383368

AN - SCOPUS:0036871435

VL - 51

SP - 1229

EP - 1237

JO - Neurosurgery

JF - Neurosurgery

SN - 0148-396X

IS - 5

ER -