Respirometry in neurons

Liang Zhang, Eugenia D Trushina

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Altered cellular energetics associated with mitochondrial dysfunction is implicated in the etiology of multiple neurodegenerative diseases emphasizing a strong need for reliable and reproducible methods of detection. The Extracellular Flux (XF) Analyzer from Seahorse Bioscience provides an outstanding opportunity for comprehensive assessment of mitochondrial function and cellular energetics by monitoring real-time aerobic respiration and glycolysis in intact, living cells. In this chapter, we provide a method to measure respiration in primary mouse cortical neurons using a Seahorse XF24 Analyzer.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages95-113
Number of pages19
Volume123
DOIs
StatePublished - 2017

Publication series

NameNeuromethods
Volume123
ISSN (Print)08932336
ISSN (Electronic)19406045

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Keywords

  • Bioenergetics
  • Electron transport
  • Mitochondria
  • Neurons
  • Oxidative phosphorylation
  • Respirometry
  • Seahorse Extracellular Flux Analyzer

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Zhang, L., & Trushina, E. D. (2017). Respirometry in neurons. In Neuromethods (Vol. 123, pp. 95-113). (Neuromethods; Vol. 123). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6890-9_5