TY - JOUR
T1 - Resolution of ambiguous low-level positive quantitative polymerase chain reaction results in TEL-AML1 positive ALL using a post-PCR fluorescent oligoligation method
AU - Chen, I. Ming
AU - Chakerian, Artemis
AU - Devidas, Meenakshi
AU - Borowitz, Michael J.
AU - Hunger, Stephen P.
AU - Willman, Cheryl L.
AU - Viswanatha, David S.
PY - 2006/11
Y1 - 2006/11
N2 - The interpretation of low-level or non-reproducible amplification results in clinical quantitative polymerase chain reaction (Q-PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low-level results might create prognostic or therapeutic dilemmas. We evaluated low-level, ambiguous Q-PCR results in a study of paired diagnostic and end-induction (day 29) TEL-AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low-level apparent Q-PCR positive results may be spurious or non-specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.
AB - The interpretation of low-level or non-reproducible amplification results in clinical quantitative polymerase chain reaction (Q-PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low-level results might create prognostic or therapeutic dilemmas. We evaluated low-level, ambiguous Q-PCR results in a study of paired diagnostic and end-induction (day 29) TEL-AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low-level apparent Q-PCR positive results may be spurious or non-specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.
KW - Fluorescent ligation detection
KW - Minimal residual disease
KW - Quantitative polymerase chain reaction
KW - TEL-AML1
UR - http://www.scopus.com/inward/record.url?scp=33749251274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749251274&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.2006.06320.x
DO - 10.1111/j.1365-2141.2006.06320.x
M3 - Article
C2 - 16984387
AN - SCOPUS:33749251274
SN - 0007-1048
VL - 135
SP - 358
EP - 361
JO - British journal of haematology
JF - British journal of haematology
IS - 3
ER -