Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury

X. Dong, S. Swaminathan, L. A. Bachman, A. J. Croatt, Karl A Nath, M. D. Griffin

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

Renal ischemia-reperfusion injury (IRI) rapidly induces production of inflammatory mediators including, and in particular, tumor necrosis factor (TNF). Possible sources include resident parenchymal and bone marrow-derived cells as well as recruited leukocytes. Cell suspensions from kidneys subjected to IRI were examined by cell separation followed by in vitro culture and enzyme-linked immunosorbent assay (ELISA), immunoperoxidase and immunofluorescence microscopy, and multicolor flow cytometry to determine the contribution of dendritic cells (DCs) to early production of TNF and other inflammatory mediators. Secretion of TNF, interleukin (IL-6), monocyte chemoattractant protein-1 (MCP-1), and regulated on activation normal T cell expressed and secreted (RANTES) was increased in cell suspensions from IRI compared with control kidneys and was higher in DC-enriched preparations. Immunostaining identified TNF+ve cells that coexpressed the DC marker CD11c. Flow cytometry of bone marrow-derived (CD45+ve) cell populations at 24 h post-IRI demonstrated that F4/80+ve/CD11c +ve DCs remained proportionately stable and exhibit higher levels of DC maturation markers, whereas the proportion of F4/80-ve DCs, monocytes, neutrophils, and T cells increased. Intracellular staining for TNF confirmed that F4/80+ve DCs were the predominant TNF+ve cell and expressed higher levels than other TNF+ve cells. In vivo depletion of DCs from the kidney substantially attenuated TNF secretion by total and CD45+ve cells following IRI. The results uncover a role for resident F4/80+ve DCs as the predominant secretors of TNF within 24 h of IRI.

Original languageEnglish (US)
Pages (from-to)619-628
Number of pages10
JournalKidney International
Volume71
Issue number7
DOIs
StatePublished - Apr 2007

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Reperfusion Injury
Dendritic Cells
Tumor Necrosis Factor-alpha
Kidney
Interleukin-6
Suspensions
Flow Cytometry
T-Lymphocytes
Cell Separation
Chemokine CCL2
Fluorescence Microscopy
Bone Marrow Cells
Monocytes
Neutrophils
Leukocytes
Bone Marrow
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Population

Keywords

  • Chemokines
  • Dendritic cells
  • Inflammation
  • Ischemia-reperfusion injury
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Nephrology

Cite this

Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. / Dong, X.; Swaminathan, S.; Bachman, L. A.; Croatt, A. J.; Nath, Karl A; Griffin, M. D.

In: Kidney International, Vol. 71, No. 7, 04.2007, p. 619-628.

Research output: Contribution to journalArticle

Dong, X. ; Swaminathan, S. ; Bachman, L. A. ; Croatt, A. J. ; Nath, Karl A ; Griffin, M. D. / Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. In: Kidney International. 2007 ; Vol. 71, No. 7. pp. 619-628.
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AB - Renal ischemia-reperfusion injury (IRI) rapidly induces production of inflammatory mediators including, and in particular, tumor necrosis factor (TNF). Possible sources include resident parenchymal and bone marrow-derived cells as well as recruited leukocytes. Cell suspensions from kidneys subjected to IRI were examined by cell separation followed by in vitro culture and enzyme-linked immunosorbent assay (ELISA), immunoperoxidase and immunofluorescence microscopy, and multicolor flow cytometry to determine the contribution of dendritic cells (DCs) to early production of TNF and other inflammatory mediators. Secretion of TNF, interleukin (IL-6), monocyte chemoattractant protein-1 (MCP-1), and regulated on activation normal T cell expressed and secreted (RANTES) was increased in cell suspensions from IRI compared with control kidneys and was higher in DC-enriched preparations. Immunostaining identified TNF+ve cells that coexpressed the DC marker CD11c. Flow cytometry of bone marrow-derived (CD45+ve) cell populations at 24 h post-IRI demonstrated that F4/80+ve/CD11c +ve DCs remained proportionately stable and exhibit higher levels of DC maturation markers, whereas the proportion of F4/80-ve DCs, monocytes, neutrophils, and T cells increased. Intracellular staining for TNF confirmed that F4/80+ve DCs were the predominant TNF+ve cell and expressed higher levels than other TNF+ve cells. In vivo depletion of DCs from the kidney substantially attenuated TNF secretion by total and CD45+ve cells following IRI. The results uncover a role for resident F4/80+ve DCs as the predominant secretors of TNF within 24 h of IRI.

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