TY - JOUR
T1 - Resident Cellular Components of the Human Lung Current Knowledge and Goals for Research on Cell Phenotyping and Function
AU - Franks, Teri J.
AU - Colby, Thomas V.
AU - Travis, William D.
AU - Tuder, Rubin M.
AU - Reynolds, Herbert Y.
AU - Brody, Arnold R.
AU - Cardoso, Wellington V.
AU - Crystal, Ronald G.
AU - Drake, Christopher J.
AU - Engelhardt, John
AU - Frid, Maria
AU - Herzog, Erica
AU - Mason, Robert
AU - Phan, Sem H.
AU - Randell, Scott H.
AU - Rose, Mary C.
AU - Stevens, Troy
AU - Serge, Julie
AU - Sunday, Mary E.
AU - Voynow, Judith A.
AU - Weinstein, Brant M.
AU - Whitsett, Jeffrey
AU - Williams, Mary C.
PY - 2008/9
Y1 - 2008/9
N2 - The purpose of the workshop was to identify still obscure or novel cellular components of the lung, to determine cell function in lung development and in health that impacts on disease, and to decide promising avenues for future research to extract and phenotype these cells. Since robust technologies are now available to identify, sort, purify, culture, and phenotype cells, progress is now within sight to unravel the origins and functional capabilities of lung cells in developmental stages and in disease. The Workshop's agenda was to first discuss the lung's embryologic development, including progenitor and stem cells, and then assess the functional and structural cells in three main compartments of the lung: (1) airway cells in bronchial and bronchiolar epithelium and bronchial glands (basal, secretory, ciliated, Clara, and neuroendocrine cells); (2) alveolar unit cells (Type 1 cells, Type 2 cells, and fibroblasts in the interstitium); and (3) pulmonary vascular cells (endothelial cells from different vascular structures, smooth muscle cells, and adventitial fibroblasts). The main recommendations were to: (1) characterize with better cell markers, both surface and nonsurface, the various cells within the lung, including progenitor cells and stem cells; (2) obtain more knowledge about gene expression in specific cell types in health and disease, which will provide insights into biological and pathologic processes; (3) develop more methodologies for cell culture, isolation, sorting, co-culture, and immortalization; and (4) promote tissue banks to facilitate the procurement of tissue from normal and from diseased lung for analysis at all levels.
AB - The purpose of the workshop was to identify still obscure or novel cellular components of the lung, to determine cell function in lung development and in health that impacts on disease, and to decide promising avenues for future research to extract and phenotype these cells. Since robust technologies are now available to identify, sort, purify, culture, and phenotype cells, progress is now within sight to unravel the origins and functional capabilities of lung cells in developmental stages and in disease. The Workshop's agenda was to first discuss the lung's embryologic development, including progenitor and stem cells, and then assess the functional and structural cells in three main compartments of the lung: (1) airway cells in bronchial and bronchiolar epithelium and bronchial glands (basal, secretory, ciliated, Clara, and neuroendocrine cells); (2) alveolar unit cells (Type 1 cells, Type 2 cells, and fibroblasts in the interstitium); and (3) pulmonary vascular cells (endothelial cells from different vascular structures, smooth muscle cells, and adventitial fibroblasts). The main recommendations were to: (1) characterize with better cell markers, both surface and nonsurface, the various cells within the lung, including progenitor cells and stem cells; (2) obtain more knowledge about gene expression in specific cell types in health and disease, which will provide insights into biological and pathologic processes; (3) develop more methodologies for cell culture, isolation, sorting, co-culture, and immortalization; and (4) promote tissue banks to facilitate the procurement of tissue from normal and from diseased lung for analysis at all levels.
KW - Cell markers
KW - Culture methods
KW - Novel cells
KW - Progenitor or stem cells
UR - http://www.scopus.com/inward/record.url?scp=50849123472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50849123472&partnerID=8YFLogxK
U2 - 10.1513/pats.200803-025HR
DO - 10.1513/pats.200803-025HR
M3 - Article
C2 - 18757314
AN - SCOPUS:50849123472
SN - 1546-3222
VL - 5
SP - 763
EP - 766
JO - Proceedings of the American Thoracic Society
JF - Proceedings of the American Thoracic Society
IS - 7
ER -