Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers

Young Soo Han, David N. Proctor, Paige C. Geiger, Gary C Sieck

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Maximum velocity of the actomyosin ATPase reaction (Vmax ATPase) and ATP consumption rate during maximum isometric activation (ATPiso) were determined in human vastus lateralis (VL) muscle fibers expressing different myosin heavy chain (MHC) isoforms. We hypothesized that the reserve capacity for ATP consumption [1 - (ratio of ATPiso to Vmax ATPase)] varies across VL muscle fibers expressing different MHC isoforms. Biopsies were obtained from 12 subjects (10 men and 2 women; age 21-66 yr). A quantitative histochemical procedure was used to measure Vmax ATPase. In permeabilized fibers, ATPiso was measured using an NADH-linked fluorometric procedure. The reserve capacity for ATP consumption was lower for fibers coexpressing MHC2x and MHC2A compared with fibers singularly expressing MHC2A and MHCslow (39 vs. 52 and 56%, respectively). Tension cost (ratio of ATPiso to generated force) also varied with fiber type, being highest in fibers coexpressing MHC2X and MHC2A. We conclude that fiber-type differences in the reserve capacity for ATP consumption and tension cost reflect functional differences such as susceptibility to fatigue.

Original languageEnglish (US)
Pages (from-to)657-664
Number of pages8
JournalJournal of Applied Physiology
Volume90
Issue number2
StatePublished - 2001

Fingerprint

Isometric Contraction
Skeletal Muscle Fibers
Adenosine Triphosphate
Adenosine Triphosphatases
Myosin Heavy Chains
Quadriceps Muscle
Protein Isoforms
Costs and Cost Analysis
Muscles
Myosins
NAD
Fatigue
Biopsy

Keywords

  • Adenosine triphosphate
  • Immunohistochemistry
  • Muscle biopsy
  • Quantitative histochemistry
  • Sodium dodecyl sulfate-polyacrylamide electrophoresis

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Han, Y. S., Proctor, D. N., Geiger, P. C., & Sieck, G. C. (2001). Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers. Journal of Applied Physiology, 90(2), 657-664.

Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers. / Han, Young Soo; Proctor, David N.; Geiger, Paige C.; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 90, No. 2, 2001, p. 657-664.

Research output: Contribution to journalArticle

Han, YS, Proctor, DN, Geiger, PC & Sieck, GC 2001, 'Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers', Journal of Applied Physiology, vol. 90, no. 2, pp. 657-664.
Han, Young Soo ; Proctor, David N. ; Geiger, Paige C. ; Sieck, Gary C. / Reserve capacity for ATP consumption during isometric contraction in human skeletal muscle fibers. In: Journal of Applied Physiology. 2001 ; Vol. 90, No. 2. pp. 657-664.
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