Requirement of the Cep57-Cep63 interaction for proper CEP152 recruitment and centriole duplication

Zhuang Wei, Tae Sung Kim, Jong Il Ahn, Lingjun Meng, Yaozong Chen, Eun Kyoung Ryu, Bonsu Ku, Ming Zhou, Seung Jun Kim, Jeong Kyu Bang, Jan M. Van Deursen, Jung Eun Park, Kyung S. Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cep57 has been characterized as a component of a pericentriolar complex containing Cep63 and Cep152. Interestingly, Cep63 and Cep152 self-assemble into a pericentriolar cylindrical architecture, and this event is critical for the orderly recruitment of Plk4, a key regulator of centriole duplication. However, the way in which Cep57 interacts with the Cep63-Cep152 complex and contributes to the structure and function of Cep63-Cep152 self-assembly remains unknown. We demonstrate that Cep57 interacts with Cep63 through N-terminal motifs and associates with Cep152 via Cep63. Three-dimensional structured illumination microscopy (3D-SIM) analyses suggested that the Cep57-Cep63-Cep152 complex is concentrically arranged around a centriole in a Cep57-in and Cep152-out manner. Cep57 mutant cells defective in Cep63 binding exhibited improper Cep63 and Cep152 localization and impaired Sas6 recruitment for procentriole assembly, proving the significance of the Cep57-Cep63 interaction. Intriguingly, Cep63 fused to a microtubule (MT)-binding domain of Cep57 functioned in concert with Cep152 to assemble around stabilized MTs in vitro. Thus, Cep57 plays a key role in architecting the Cep63-Cep152 assembly around centriolar MTs and promoting centriole biogenesis. This study may offer a platform to investigate how the organization and function of the pericentriolar architecture are altered by disease-associated mutations found in the Cep57-Cep63-Cep152 complex.

Original languageEnglish (US)
Article number00535-19
JournalMolecular and cellular biology
Volume40
Issue number10
DOIs
StatePublished - May 1 2020

Keywords

  • Centriole biogenesis
  • Cep152
  • Cep57
  • Cep63
  • PCM

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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