Reprogramming the pluripotent cell cycle: Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells

Prachi N. Ghule, Ricardo Medina, Christopher J. Lengner, Matthew Mandeville, Meng Qiao, Zbigniew Dominski, Jane B. Lian, Janet L. Stein, Andre J van Wijnen, Gary S. Stein

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells derived from terminally differentiated human fibroblasts are reprogrammed to possess stem cell like properties. However, the extent to which iPS cells exhibit unique properties of the human embryonic stem (hES) cell cycle remains to be established. hES cells are characterized by an abbreviated G1 phase (~2.5h) and accelerated organization of subnuclear domains that mediate the assembly of regulatory machinery for histone gene expression [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters of iPS cells in comparison to hES cells. Analysis of DNA synthesis [5-bromo-2'-deoxy-uridine (BrdU) incorporation], cell cycle distribution (FACS analysis and Ki67 staining) and subnuclear organization of HLBs [immunofluorescence microscopy and fluorescence in situ hybridization (FISH)] revealed that human iPS cells have a short G1 phase (~2.5h) and an abbreviated cell cycle (16-18h). Furthermore, HLBs are formed and reorganized rapidly after mitosis (within 1.5-2h). Thus, reprogrammed iPS cells have cell cycle kinetics and dynamic subnuclear organization of regulatory machinery that are principal properties of pluripotent hES cells. Our findings support the concept that the abbreviated cell cycle of hES and iPS cells is functionally linked to pluripotency.

Original languageEnglish (US)
Pages (from-to)1149-1156
Number of pages8
JournalJournal of Cellular Physiology
Volume226
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
G1 Phase
Stem cells
Restoration
Cell Cycle
Cells
Histones
Bromodeoxyuridine
Fluorescence In Situ Hybridization
Fluorescence Microscopy
Machinery
Mitosis
Stem Cells
Fibroblasts
Staining and Labeling
Gene Expression
Human Embryonic Stem Cells
DNA
Gene expression
Microscopic examination

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Ghule, P. N., Medina, R., Lengner, C. J., Mandeville, M., Qiao, M., Dominski, Z., ... Stein, G. S. (2011). Reprogramming the pluripotent cell cycle: Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. Journal of Cellular Physiology, 226(5), 1149-1156. https://doi.org/10.1002/jcp.22440

Reprogramming the pluripotent cell cycle : Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. / Ghule, Prachi N.; Medina, Ricardo; Lengner, Christopher J.; Mandeville, Matthew; Qiao, Meng; Dominski, Zbigniew; Lian, Jane B.; Stein, Janet L.; van Wijnen, Andre J; Stein, Gary S.

In: Journal of Cellular Physiology, Vol. 226, No. 5, 05.2011, p. 1149-1156.

Research output: Contribution to journalArticle

Ghule, PN, Medina, R, Lengner, CJ, Mandeville, M, Qiao, M, Dominski, Z, Lian, JB, Stein, JL, van Wijnen, AJ & Stein, GS 2011, 'Reprogramming the pluripotent cell cycle: Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells', Journal of Cellular Physiology, vol. 226, no. 5, pp. 1149-1156. https://doi.org/10.1002/jcp.22440
Ghule, Prachi N. ; Medina, Ricardo ; Lengner, Christopher J. ; Mandeville, Matthew ; Qiao, Meng ; Dominski, Zbigniew ; Lian, Jane B. ; Stein, Janet L. ; van Wijnen, Andre J ; Stein, Gary S. / Reprogramming the pluripotent cell cycle : Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. In: Journal of Cellular Physiology. 2011 ; Vol. 226, No. 5. pp. 1149-1156.
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