Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors

Hui Cheng, Heather Yin Kuan Ang, Chadi A. El Farran, Pin Li, Hai Tong Fang, Tong Ming Liu, Say Li Kong, Michael Lingzi Chin, Wei Yin Ling, Edwin Kok Hao Lim, Hu Li, Tara Huber, Kyle M. Loh, Yuin Han Loh, Bing Lim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recent efforts have attempted to convert non-blood cells into hematopoietic stem cells (HSCs) with the goal of generating blood lineages de novo. Here we show that hematopoietic transcription factors Scl, Lmo2, Runx1 and Bmi1 can convert a developmentally distant lineage (fibroblasts) into 'induced hematopoietic progenitors' (iHPs). Functionally, iHPs generate acetylcholinesterase+ megakaryocytes and phagocytic myeloid cells in vitro and can also engraft immunodeficient mice, generating myeloerythoid and B-lymphoid cells for up to 4 months in vivo. Molecularly, iHPs transcriptionally resemble native Kit+ hematopoietic progenitors. Mechanistically, reprogramming factor Lmo2 implements a hematopoietic programme in fibroblasts by rapidly binding to and upregulating the Hhex and Gfi1 genes within days. Moreover the reprogramming transcription factors also require extracellular BMP and MEK signalling to cooperatively effectuate reprogramming. Thus, the transcription factors that orchestrate embryonic hematopoiesis can artificially reconstitute this programme in developmentally distant fibroblasts, converting them into engraftable blood progenitors.

Original languageEnglish (US)
Article number13396
JournalNature Communications
Volume7
DOIs
StatePublished - Nov 21 2016

Fingerprint

fibroblasts
Fibroblasts
mice
Transcription Factors
Blood
blood
Megakaryocytes
hematopoiesis
Mitogen-Activated Protein Kinase Kinases
Hematopoiesis
Myeloid Cells
Acetylcholinesterase
Phagocytes
Hematopoietic Stem Cells
Stem cells
kits
stem cells
Genes
cells
Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Cheng, H., Ang, H. Y. K., El Farran, C. A., Li, P., Fang, H. T., Liu, T. M., ... Lim, B. (2016). Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors. Nature Communications, 7, [13396]. https://doi.org/10.1038/ncomms13396

Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors. / Cheng, Hui; Ang, Heather Yin Kuan; El Farran, Chadi A.; Li, Pin; Fang, Hai Tong; Liu, Tong Ming; Kong, Say Li; Chin, Michael Lingzi; Ling, Wei Yin; Lim, Edwin Kok Hao; Li, Hu; Huber, Tara; Loh, Kyle M.; Loh, Yuin Han; Lim, Bing.

In: Nature Communications, Vol. 7, 13396, 21.11.2016.

Research output: Contribution to journalArticle

Cheng, H, Ang, HYK, El Farran, CA, Li, P, Fang, HT, Liu, TM, Kong, SL, Chin, ML, Ling, WY, Lim, EKH, Li, H, Huber, T, Loh, KM, Loh, YH & Lim, B 2016, 'Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors', Nature Communications, vol. 7, 13396. https://doi.org/10.1038/ncomms13396
Cheng, Hui ; Ang, Heather Yin Kuan ; El Farran, Chadi A. ; Li, Pin ; Fang, Hai Tong ; Liu, Tong Ming ; Kong, Say Li ; Chin, Michael Lingzi ; Ling, Wei Yin ; Lim, Edwin Kok Hao ; Li, Hu ; Huber, Tara ; Loh, Kyle M. ; Loh, Yuin Han ; Lim, Bing. / Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors. In: Nature Communications. 2016 ; Vol. 7.
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