Reproducibility of measurements of regional myocardial blood flow in a model of coronary artery disease: Comparison of H2 15O and 13NH3 PET techniques

19 Scopus citations

Abstract

PET absolute myocardial blood flow (MBF) with H2 15O and 13NH3 are widely used in clinical and research settings. However, their reproducibility with a 16-myocardial segment model has not been examined in chronic coronary artery disease (CAD). We examined the short-term reproducibility of PET H2 15O MBF and PET 13NH3 MBF in an animal model of chronic CAD. Methods: Twelve swine (mean weight ± SD, 38 ± 5 kg) underwent percutaneous placement of a copper stent in the mid circumflex coronary artery, resulting in an intense inflammatory fibrotic reaction with luminal stenosis at 4 wk. Each animal underwent repeated resting MBF measurements by PET H2 15O and PET 13NH3. Attenuation-corrected images were analyzed using commercial software to yield absolute MBF (mL/min/g) in 16 myocardial segments. MBF was also normalized to the rate·pressure product (RPP). Results: By Bland-Altman reproducibility plots, the mean difference was 0.01 ± 0.18 mL/min/g and 0.01 ± 0.11 mL/min/g, with confidence limits of ±0.36 and ±0.22 mL/min/g for uncorrected regional PET H2 15O MBF and for uncorrected regional PET 13NH3 MBF, respectively. The repeatability coefficient ranged from 0.09 to 0.43 mL/min/g for H2 15O and from 0.09 to 0.18 mL/min/g for 13NH3 regional MBF. RPP correction did not improve reproducibility for either PET H2 15O or PET 13NH3 MBF. The mean difference in PET H 2 15O MBF was 0.03 ± 0.14 mL/min/g and 0.02 ± 0.19 mL/min/g for infarcted and remote regions, respectively, and in PET 13NH3 MBF was 0.03 ± 0.11 mL/min/g and 0.00 ± 0.09 mL/min/g for infarcted and remote regions, respectively. Conclusion: PET H2 15O and PET 13NH3 resting MBF showed excellent reproducibility in a closed-chest animal model of chronic CAD. Resting PET 13NH3 MBF was more reproducible than resting PET H2 15O MBF. A high level of reproducibility was maintained in areas of lower flow with infarction for both isotopes.

Original languageEnglish (US)
Pages (from-to)1193-1201
Number of pages9
JournalJournal of Nuclear Medicine
Volume47
Issue number7
StatePublished - Jul 1 2006

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Keywords

  • Blood flow
  • Coronary disease
  • Imaging
  • PET
  • Radioisotopes

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Chareonthaitawee, P., Christenson, S. D., Anderson, J. L., Kemp, B. J., Hodge, D. O., Ritman, E. L., & Gibbons, R. J. (2006). Reproducibility of measurements of regional myocardial blood flow in a model of coronary artery disease: Comparison of H2 15O and 13NH3 PET techniques. Journal of Nuclear Medicine, 47(7), 1193-1201.