Reproducibility of measurements of regional myocardial blood flow in a model of coronary artery disease: Comparison of H215O and 13NH3 PET techniques

Panithaya Chareonthaitawee, Stuart D. Christenson, Jill L. Anderson, Brad J. Kemp, David O. Hodge, Erik L. Ritman, Raymond J. Gibbons

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

PET absolute myocardial blood flow (MBF) with H215O and 13NH3 are widely used in clinical and research settings. However, their reproducibility with a 16-myocardial segment model has not been examined in chronic coronary artery disease (CAD). We examined the short-term reproducibility of PET H215O MBF and PET 13NH3 MBF in an animal model of chronic CAD. Methods: Twelve swine (mean weight ± SD, 38 ± 5 kg) underwent percutaneous placement of a copper stent in the mid circumflex coronary artery, resulting in an intense inflammatory fibrotic reaction with luminal stenosis at 4 wk. Each animal underwent repeated resting MBF measurements by PET H2 15O and PET 13NH3. Attenuation-corrected images were analyzed using commercial software to yield absolute MBF (mL/min/g) in 16 myocardial segments. MBF was also normalized to the rate·pressure product (RPP). Results: By Bland-Altman reproducibility plots, the mean difference was 0.01 ± 0.18 mL/min/g and 0.01 ± 0.11 mL/min/g, with confidence limits of ±0.36 and ±0.22 mL/min/g for uncorrected regional PET H215O MBF and for uncorrected regional PET 13NH3 MBF, respectively. The repeatability coefficient ranged from 0.09 to 0.43 mL/min/g for H215O and from 0.09 to 0.18 mL/min/g for 13NH3 regional MBF. RPP correction did not improve reproducibility for either PET H215O or PET 13NH3 MBF. The mean difference in PET H 215O MBF was 0.03 ± 0.14 mL/min/g and 0.02 ± 0.19 mL/min/g for infarcted and remote regions, respectively, and in PET 13NH3 MBF was 0.03 ± 0.11 mL/min/g and 0.00 ± 0.09 mL/min/g for infarcted and remote regions, respectively. Conclusion: PET H215O and PET 13NH3 resting MBF showed excellent reproducibility in a closed-chest animal model of chronic CAD. Resting PET 13NH3 MBF was more reproducible than resting PET H215O MBF. A high level of reproducibility was maintained in areas of lower flow with infarction for both isotopes.

Original languageEnglish (US)
Pages (from-to)1193-1201
Number of pages9
JournalJournal of Nuclear Medicine
Volume47
Issue number7
StatePublished - Jul 1 2006

Keywords

  • Blood flow
  • Coronary disease
  • Imaging
  • PET
  • Radioisotopes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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    Chareonthaitawee, P., Christenson, S. D., Anderson, J. L., Kemp, B. J., Hodge, D. O., Ritman, E. L., & Gibbons, R. J. (2006). Reproducibility of measurements of regional myocardial blood flow in a model of coronary artery disease: Comparison of H215O and 13NH3 PET techniques. Journal of Nuclear Medicine, 47(7), 1193-1201.