Reproducibility of global and local reconstruction of three-dimensional micro-computed tomography of iliac crest biopsies

Steven M. Jorgensen, Diane R. Eaker, Andrew J. Vercnocke, Erik L. Ritman

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Variation in computed tomography (CT) image gray-scale and spatial geometry due to specimen orientation, magnification, voxel size, differences in X-ray photon energy and limited field-of-view during the scan, were evaluated in repeated micro-CT scans of iliac crest biopsies and test phantoms. Using the micro-CT scanner on beamline X2B at the Brookhaven National Laboratory's National Synchrotron Light Source, 3-D micro-CT images were generated. They consisted of up to 1024 × 2400 2, 4-, cubic voxels, each with 16-bit gray-scale. We also reconstructed the images at 16-, 32-, and 48- voxel resolution. Scan data were reconstructed from the complete profiles using filtered back-projection and from truncated profiles using profile-extension and with a Local reconstruction algorithm. Three biopsies and one bone-like test phantom were each rescanned at three different times at annual intervals. For the full-data-set reconstructions, the reproducibility of the estimates of mineral content of bone at mean bone opacity value, was ±28.8 mg/cm 3, i.e., 2.56%, in a 4-mu cubic voxel at the 95% confidence level. The reproducibility decreased with increased voxel size. The interscan difference in imaged bone volume ranged from 0.86 ± 0.64% at 4-mu voxel resolution, and 2.64 ± 2.48% at 48-mu.

Original languageEnglish (US)
Article number4384612
Pages (from-to)569-575
Number of pages7
JournalIEEE transactions on medical imaging
Issue number4
StatePublished - Apr 2008


  • Bone
  • Mineralization
  • Photon energy
  • Voxel size

ASJC Scopus subject areas

  • Software
  • Radiological and Ultrasound Technology
  • Computer Science Applications
  • Electrical and Electronic Engineering


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