TY - JOUR
T1 - Reproducibility of global and local reconstruction of three-dimensional micro-computed tomography of iliac crest biopsies
AU - Jorgensen, Steven M.
AU - Eaker, Diane R.
AU - Vercnocke, Andrew J.
AU - Ritman, Erik L.
N1 - Funding Information:
Manuscript received July 25, 2007; revised October 29, 2007. This work was supported in part by the National Institutes of Health (NIH) under Grant EB000305 and in part by the Alliance for Better Bone Health (Procter and Gamble Pharmaceuticals and Sanofi-Aventis). The authors acknowledge the image acquisition carried out at the National Synchrotron Light Source, Brookhaven National Laboratory, which is supported by the U.S. Department of Energy, Division of Materials Sciences and Division of Chemical Sciences under Contract DE-AC02-98CH10886. Asterisk indicates corresponding author.
PY - 2008/4
Y1 - 2008/4
N2 - Variation in computed tomography (CT) image gray-scale and spatial geometry due to specimen orientation, magnification, voxel size, differences in X-ray photon energy and limited field-of-view during the scan, were evaluated in repeated micro-CT scans of iliac crest biopsies and test phantoms. Using the micro-CT scanner on beamline X2B at the Brookhaven National Laboratory's National Synchrotron Light Source, 3-D micro-CT images were generated. They consisted of up to 1024 × 2400 2, 4-, cubic voxels, each with 16-bit gray-scale. We also reconstructed the images at 16-, 32-, and 48- voxel resolution. Scan data were reconstructed from the complete profiles using filtered back-projection and from truncated profiles using profile-extension and with a Local reconstruction algorithm. Three biopsies and one bone-like test phantom were each rescanned at three different times at annual intervals. For the full-data-set reconstructions, the reproducibility of the estimates of mineral content of bone at mean bone opacity value, was ±28.8 mg/cm 3, i.e., 2.56%, in a 4-mu cubic voxel at the 95% confidence level. The reproducibility decreased with increased voxel size. The interscan difference in imaged bone volume ranged from 0.86 ± 0.64% at 4-mu voxel resolution, and 2.64 ± 2.48% at 48-mu.
AB - Variation in computed tomography (CT) image gray-scale and spatial geometry due to specimen orientation, magnification, voxel size, differences in X-ray photon energy and limited field-of-view during the scan, were evaluated in repeated micro-CT scans of iliac crest biopsies and test phantoms. Using the micro-CT scanner on beamline X2B at the Brookhaven National Laboratory's National Synchrotron Light Source, 3-D micro-CT images were generated. They consisted of up to 1024 × 2400 2, 4-, cubic voxels, each with 16-bit gray-scale. We also reconstructed the images at 16-, 32-, and 48- voxel resolution. Scan data were reconstructed from the complete profiles using filtered back-projection and from truncated profiles using profile-extension and with a Local reconstruction algorithm. Three biopsies and one bone-like test phantom were each rescanned at three different times at annual intervals. For the full-data-set reconstructions, the reproducibility of the estimates of mineral content of bone at mean bone opacity value, was ±28.8 mg/cm 3, i.e., 2.56%, in a 4-mu cubic voxel at the 95% confidence level. The reproducibility decreased with increased voxel size. The interscan difference in imaged bone volume ranged from 0.86 ± 0.64% at 4-mu voxel resolution, and 2.64 ± 2.48% at 48-mu.
KW - Bone
KW - Mineralization
KW - Photon energy
KW - Voxel size
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U2 - 10.1109/TMI.2007.912821
DO - 10.1109/TMI.2007.912821
M3 - Article
C2 - 18390353
AN - SCOPUS:41649106931
SN - 0278-0062
VL - 27
SP - 569
EP - 575
JO - IEEE Transactions on Medical Imaging
JF - IEEE Transactions on Medical Imaging
IS - 4
M1 - 4384612
ER -