@article{83f29ba4743d4628a4d51207b982b6e1,
title = "Reproducibility of cerebral cortical infarction in the wistar rat after middle cerebral artery occlusion",
abstract = "Although middle cerebral artery (MCA) occlusion in the rat is often used to study focal cerebral ischemia, the model of ischemia affects the size and reproducibility of infarction. The purpose of this experiment was to methodically examine different preparations to determine the optimum focal cerebral ischemia model to produce a reproducible severe ischemic injury. Eighty-two Wistar rats underwent either 1 hour, 3 hour, or permanent MCA occlusion combined with no, unilateral, or bilateral common carotid artery artery (CCA) occlusion. Three days after ischemia, the animals were prepared for tetrazolium chloride assessment of infarction size. One-hour MCA occlusion produced a coefficient of variation (CV) of 200% with an infarction volume of 20.3±10.5 mm3. Adding unilateral or bilateral CCA occlusion resulted in a CV of 134% and 101%, respectively. Three-hour MCA occlusion combined with bilateral CCA occlusion decreased the CV to 58% with a cortical infarction volume of 82.6±12.1 mm3, P<05, compared with 1-hour MCA occlusion with or without CCA occlusion. Permanent MCA occlusion combined with 3 hours of bilateral CCA occlusion resulted in a CV of 47% with a cortical infarction volume of 89.6±16.0 mm3. These results indicate that 3-hour MCA occlusion combined with bilateral CCA occlusion provide consistently a large infarction volume after temporary focal cerebral ischemia.",
keywords = "Focal ischemia, Infarction, Model, Stroke, Therapy, Wistar rat",
author = "Coert, {Bert A.} and Anderson, {Robert E.} and Meyer, {Fredric B.}",
note = "Funding Information: Numerous experimental animal models have been developed to study the pathophysiology and to test therapeutic interventions in focal cerebral ischemia. Rodent models have gained considerable interest for a variety of reasons including their low cost and wide availability, and new possibilities with genetically altered animals. Because of Tamura et al's 1 description of a middle cerebral artery occlusion (MCAO) technique, MCAO in rats has been widely used as a model of focal cerebral ischemia. Unfortunately, there is significant variability in the reproducibility and size of cortical infarction using the MCAO model in Wistar rats.Z3 This makes it problematic to investigate the pathophysiology and to From the Thoralf M. Sundt, Jr, MD Neurosurgical Research Laboratory, Mayo Clinic, Rochester, MN. Received July 1,1998; accepted November 19,1998. Supported by National Institute of Health grant RO1-25374. Address reprint requests to Fredric B. Meyer, MD, Department of Neurosurgery, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Copyright 9 1999 by National Stroke Association 1052-3057/99/0806-000353.00/0 compare the efficacy of therapeutic interventions. This variability or inconsistency can be attributed to the model (location and duration of occlusion), strain/vendor, 4,5 age/weight, 6,7 anesthesia, s brain and body temperature, 9 systemic parameters (Pao2, Paco2, and pH); blood pressure l~ and serum glucose levels (Table 1). 12-14 The purpose of this experiment was to rigorously examine various models of temporary focal cerebral ischemia in the Wistar rat to improve consistency and reproducibility of cortical infarction.",
year = "1999",
month = nov,
doi = "10.1016/S1052-3057(99)80045-7",
language = "English (US)",
volume = "8",
pages = "380--387",
journal = "Journal of Stroke and Cerebrovascular Diseases",
issn = "1052-3057",
publisher = "W.B. Saunders Ltd",
number = "6",
}