Representative cDNA libraries and their utility in gene expression profiling

Wilson O. Endege, K. E. Steinmann, L. A. Boardman, S. N. Thibodeau, R. Schlegel

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

An increasing interest in gene expression profiles in human diseases has led to the use of microdissected tumors and biopsies in gene discovery approaches. Since many of these clinical samples yield extremely small amounts of RNA, reproducible methods are needed to amplify this RNA while maintaining the original message profile. Using the SMART(TM) cDNA Synthesis Method, we show that high-, medium- and low-abundance transcripts can be amplified in a representative fashion and that the resulting cDNA can also be used as a complex probe to confirm gene expression differences identified by other techniques.

Original languageEnglish (US)
Pages (from-to)542-550
Number of pages9
JournalBioTechniques
Volume26
Issue number3
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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