Replication of associations between cytokine and cytokine receptor single nucleotide polymorphisms and measles-specific adaptive immunophenotypic extremes

Sarah J. White, Iana H. Haralambieva, Inna G. Ovsyannikova, Robert A. Vierkant, Megan M. O'Byrne, Gregory A. Poland

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Our objective was to replicate previously reported associations between cytokine and cytokine receptor SNPs and humoral and CMI (cell-mediated immune) responses to measles vaccine. All subjects (n=758) received two doses of MMR (measles/mumps/rubella) vaccine. From these subjects, candidate cytokine and cytokine receptor SNPs were genotyped and analyzed in 29-30 subjects falling into one of four "extreme" humoral (Abhigh/low) and CMI (CMIhigh/low) response quadrants. Associations between seven SNPs (out of 11 in the discovery study) and measles-specific neutralizing antibody levels and IFN-γ ELISPOT responses were evaluated using chi-square tests. We found one replicated association for SNP rs372889 in the IL12RB1 gene (P=0.03 for AbhighCMIhigh vs. AblowCMIlow). Our findings demonstrate the importance of replicating genotypic-phenotypic associations, which can be achieved using immunophenotypic extremes and smaller sample sizes. We speculate that IL12RB1 polymorphisms may affect IL-12 and IL-23 binding and downstream effects, which are critical cytokines in the CMI response to measles vaccine.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalHuman Immunology
Volume73
Issue number6
DOIs
StatePublished - Jun 1 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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