Repetitive DNA (TGGA)n 5′ to the human myelin basic protein gene: A new form of oligonucleotide repetitive sequence showing length polymorphism

Kevin B. Boylan, T. Merrill Ayres, Brian Popko, Naoki Takahashi, Leroy E. Hood, Stanley B. Prusiner

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

DNA 5′ to the human myelin basic protein (MBP) gene, mapped to 18q22 → qter, is known to manifest multiallelic DNA length variation with heterozygosity of at least 45%. Isolation of genomic DNA containing the MBP gene first exon and its 5′ flanking region reveals that this polymorphism arises from a 994-bp region of the diverged tandem repeat (TGGA)249. This sequence is located from 1082 to 2075 bp upstream of the MBP initiator methionine. The repetitive sequence is 18% diverged from (TGGA)249 and from analysis of higher order subsequence reiterations appears to have undergone extensive recombination. The pattern of higher order repetition suggests that multiple cross-over and gene conversion events have occurred within a 1.0-kb region. Molecular clones of this sequence represent essentially the longest allelic form of this region seen in Southern transfer analysis. This repetitive DNA is similar to a sequence 5′ to the human myoglobin gene.

Original languageEnglish (US)
Pages (from-to)16-22
Number of pages7
JournalGenomics
Volume6
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

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Nucleic Acid Repetitive Sequences
Oligonucleotides
Myelin Basic Protein
DNA
Genes
Gene Conversion
Tandem Repeat Sequences
Myoglobin
5' Flanking Region
Methionine
Genetic Recombination
Exons
Clone Cells
human MBP protein

ASJC Scopus subject areas

  • Genetics

Cite this

Repetitive DNA (TGGA)n 5′ to the human myelin basic protein gene : A new form of oligonucleotide repetitive sequence showing length polymorphism. / Boylan, Kevin B.; Merrill Ayres, T.; Popko, Brian; Takahashi, Naoki; Hood, Leroy E.; Prusiner, Stanley B.

In: Genomics, Vol. 6, No. 1, 1990, p. 16-22.

Research output: Contribution to journalArticle

Boylan, Kevin B. ; Merrill Ayres, T. ; Popko, Brian ; Takahashi, Naoki ; Hood, Leroy E. ; Prusiner, Stanley B. / Repetitive DNA (TGGA)n 5′ to the human myelin basic protein gene : A new form of oligonucleotide repetitive sequence showing length polymorphism. In: Genomics. 1990 ; Vol. 6, No. 1. pp. 16-22.
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