Abstract
DNA 5′ to the human myelin basic protein (MBP) gene, mapped to 18q22 → qter, is known to manifest multiallelic DNA length variation with heterozygosity of at least 45%. Isolation of genomic DNA containing the MBP gene first exon and its 5′ flanking region reveals that this polymorphism arises from a 994-bp region of the diverged tandem repeat (TGGA)249. This sequence is located from 1082 to 2075 bp upstream of the MBP initiator methionine. The repetitive sequence is 18% diverged from (TGGA)249 and from analysis of higher order subsequence reiterations appears to have undergone extensive recombination. The pattern of higher order repetition suggests that multiple cross-over and gene conversion events have occurred within a 1.0-kb region. Molecular clones of this sequence represent essentially the longest allelic form of this region seen in Southern transfer analysis. This repetitive DNA is similar to a sequence 5′ to the human myoglobin gene.
Original language | English (US) |
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Pages (from-to) | 16-22 |
Number of pages | 7 |
Journal | Genomics |
Volume | 6 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1990 |
ASJC Scopus subject areas
- Genetics