Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo; Stefania Niada; Elena Arrigoni; Alessia Di Giancamillo; Cinzia Domeneghini; Mahrokh Dadsetan; Michael J. Yaszemski; Dario Gastaldi; Pasquale Vena; Matteo Taffetani; Alberto Zerbi; Valerio Sansone; Giuseppe M. Peretti; Anna T. Brini

doi:10.2217/rme.14.77

Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo, Stefania Niada, Elena Arrigoni, Alessia Di Giancamillo, Cinzia Domeneghini, Mahrokh Dadsetan, Michael J. Yaszemski, Dario Gastaldi, Pasquale Vena, Matteo Taffetani, Alberto Zerbi, Valerio Sansone, Giuseppe M. Peretti, Anna T. Brini

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

Original language	English (US)
Pages (from-to)	135-151
Number of pages	17
Journal	Regenerative Medicine
Volume	10
Issue number	2
DOIs	https://doi.org/10.2217/rme.14.77
State	Published - Mar 1 2015

Keywords

adipose-derived stem cells
bone
cartilage
oligo(polyethylene glycol)fumarate hydrogel
osteochondral defects

ASJC Scopus subject areas

Biomedical Engineering
Embryology

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10.2217/rme.14.77

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De Girolamo, L., Niada, S., Arrigoni, E., Di Giancamillo, A., Domeneghini, C., Dadsetan, M., Yaszemski, M. J., Gastaldi, D., Vena, P., Taffetani, M., Zerbi, A., Sansone, V., Peretti, G. M., & Brini, A. T. (2015). Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. Regenerative Medicine, 10(2), 135-151. https://doi.org/10.2217/rme.14.77

De Girolamo, L, Niada, S, Arrigoni, E, Di Giancamillo, A, Domeneghini, C, Dadsetan, M, Yaszemski, MJ, Gastaldi, D, Vena, P, Taffetani, M, Zerbi, A, Sansone, V, Peretti, GM & Brini, AT 2015, 'Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells', Regenerative Medicine, vol. 10, no. 2, pp. 135-151. https://doi.org/10.2217/rme.14.77

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title = "Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells",

abstract = "Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.",

keywords = "adipose-derived stem cells, bone, cartilage, oligo(polyethylene glycol)fumarate hydrogel, osteochondral defects",

author = "{De Girolamo}, Laura and Stefania Niada and Elena Arrigoni and {Di Giancamillo}, Alessia and Cinzia Domeneghini and Mahrokh Dadsetan and Yaszemski, {Michael J.} and Dario Gastaldi and Pasquale Vena and Matteo Taffetani and Alberto Zerbi and Valerio Sansone and Peretti, {Giuseppe M.} and Brini, {Anna T.}",

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doi = "10.2217/rme.14.77",

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AU - De Girolamo, Laura

AU - Niada, Stefania

AU - Arrigoni, Elena

AU - Di Giancamillo, Alessia

AU - Domeneghini, Cinzia

AU - Dadsetan, Mahrokh

AU - Yaszemski, Michael J.

AU - Gastaldi, Dario

AU - Vena, Pasquale

AU - Taffetani, Matteo

AU - Zerbi, Alberto

AU - Sansone, Valerio

AU - Peretti, Giuseppe M.

AU - Brini, Anna T.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

AB - Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

KW - adipose-derived stem cells

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KW - cartilage

KW - oligo(polyethylene glycol)fumarate hydrogel

KW - osteochondral defects

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Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Abstract

Keywords

ASJC Scopus subject areas

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