Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo, Stefania Niada, Elena Arrigoni, Alessia Di Giancamillo, Cinzia Domeneghini, Mahrokh Dadsetan, Michael J Yaszemski, Dario Gastaldi, Pasquale Vena, Matteo Taffetani, Alberto Zerbi, Valerio Sansone, Giuseppe M. Peretti, Anna T. Brini

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

Original languageEnglish (US)
Pages (from-to)135-151
Number of pages17
JournalRegenerative Medicine
Volume10
Issue number2
DOIs
StatePublished - Mar 1 2015

Keywords

  • adipose-derived stem cells
  • bone
  • cartilage
  • oligo(polyethylene glycol)fumarate hydrogel
  • osteochondral defects

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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