Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells

Laura De Girolamo, Stefania Niada, Elena Arrigoni, Alessia Di Giancamillo, Cinzia Domeneghini, Mahrokh Dadsetan, Michael J Yaszemski, Dario Gastaldi, Pasquale Vena, Matteo Taffetani, Alberto Zerbi, Valerio Sansone, Giuseppe M. Peretti, Anna T. Brini

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aim: Critical knee osteochondral defects in seven adult minipigs were treated with oligo(polyethylene glycol)fumarate (OPF) hydrogel combined with autologous or human adipose-derived stem cells (ASCs), and evaluated after 6 months. Methods: Four defects were made on the peripheral part of right trochleas (n = 28), and treated with OPF scaffold alone or pre-seeded with ASCs. Results: A better quality cartilage tissue characterized by improved biomechanical properties and higher collagen type II expression was observed in the defects treated by autologous or human ASC-loaded OPF; similarly this approach induced the regeneration of more mature bone with upregulation of collagen type I expression. Conclusion: This study provides the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel allow improving osteochondral defect regeneration in a minipig model.

Original languageEnglish (US)
Pages (from-to)135-151
Number of pages17
JournalRegenerative Medicine
Volume10
Issue number2
DOIs
StatePublished - Mar 1 2015

Fingerprint

Miniature Swine
Hydrogel
Stem cells
Mesenchymal Stromal Cells
Hydrogels
Repair
Stem Cells
Defects
Collagen
Regeneration
Fumarates
Collagen Type II
Cartilage
Collagen Type I
Scaffolds
Polyethylene glycols
Knee
Bone
Up-Regulation
Swine

Keywords

  • adipose-derived stem cells
  • bone
  • cartilage
  • oligo(polyethylene glycol)fumarate hydrogel
  • osteochondral defects

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

De Girolamo, L., Niada, S., Arrigoni, E., Di Giancamillo, A., Domeneghini, C., Dadsetan, M., ... Brini, A. T. (2015). Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. Regenerative Medicine, 10(2), 135-151. https://doi.org/10.2217/rme.14.77

Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. / De Girolamo, Laura; Niada, Stefania; Arrigoni, Elena; Di Giancamillo, Alessia; Domeneghini, Cinzia; Dadsetan, Mahrokh; Yaszemski, Michael J; Gastaldi, Dario; Vena, Pasquale; Taffetani, Matteo; Zerbi, Alberto; Sansone, Valerio; Peretti, Giuseppe M.; Brini, Anna T.

In: Regenerative Medicine, Vol. 10, No. 2, 01.03.2015, p. 135-151.

Research output: Contribution to journalArticle

De Girolamo, L, Niada, S, Arrigoni, E, Di Giancamillo, A, Domeneghini, C, Dadsetan, M, Yaszemski, MJ, Gastaldi, D, Vena, P, Taffetani, M, Zerbi, A, Sansone, V, Peretti, GM & Brini, AT 2015, 'Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells', Regenerative Medicine, vol. 10, no. 2, pp. 135-151. https://doi.org/10.2217/rme.14.77
De Girolamo L, Niada S, Arrigoni E, Di Giancamillo A, Domeneghini C, Dadsetan M et al. Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. Regenerative Medicine. 2015 Mar 1;10(2):135-151. https://doi.org/10.2217/rme.14.77
De Girolamo, Laura ; Niada, Stefania ; Arrigoni, Elena ; Di Giancamillo, Alessia ; Domeneghini, Cinzia ; Dadsetan, Mahrokh ; Yaszemski, Michael J ; Gastaldi, Dario ; Vena, Pasquale ; Taffetani, Matteo ; Zerbi, Alberto ; Sansone, Valerio ; Peretti, Giuseppe M. ; Brini, Anna T. / Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesenchymal stem cells. In: Regenerative Medicine. 2015 ; Vol. 10, No. 2. pp. 135-151.
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