Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway

Katie Twigger, Victoria Roulstone, Joan Kyula, Eleni M. Karapanagiotou, Konstantinos N. Syrigos, Richard Morgan, Christine White, Shreerang Bhide, Gerard Nuovo, Matt Coffey, Brad Thompson, Adel Jebar, Fiona Errington, Alan A. Melcher, Richard Geoffrey Vile, Hardev S. Pandha, Kevin J. Harrington

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Reovirus exploits aberrant signalling downstream of Ras to mediate tumor-specific oncolysis. Since ~90% squamous cell carcinomas of the head and neck (SCCHN) over-express EGFR and SCCHN cell lines are sensitive to oncolytic reovirus, we conducted a detailed analysis of the effects of reovirus in 15 head and neck cancer cell lines. Both pre- and post-entry events were studied in an attempt to define biomarkers predictive of sensitivity/resistance to reovirus. In particular, we analysed the role of EGFR/Ras signalling in determining virus-mediated cytotoxicity in SCCHN.Methods: To test whether EGFR pathway activity was predictive of increased sensitivity to reovirus, correlative analyses between reoviral IC50 by MTT assay and EGFR levels by western blot and FACS were conducted. Inhibition or stimulation of EGFR signalling were analysed for their effect on reoviral oncolysis by MTT assay, and viral growth by TCID50 assay. We next analysed the effects of inhibiting signalling downstream of Ras, by specific inhibitors of p38MAPK, PI3-K or MEK, on reoviral killing examined by MTT assay. The role of PKR in reoviral killing was also determined by blockade of PKR using 2-aminopurine and assaying for cell survival by MTT assay. The apoptotic response of SCCHN to reovirus was examined by western blot analysis of caspase 3 cleavage.Results: Correlative analyses between reoviral sensitivity and EGFR levels revealed no association. Intermediate sub-viral and core particles showed the same infectivity/cytotoxicity as intact reovirus. Therefore, sensitivity was not determined by cell entry. In 4 cell lines, oncolysis and viral growth were both unaffected by inhibition or stimulation of EGFR signalling. Inhibition of signalling downstream of Ras did not abrogate reoviral oncolysis and, in addition, modulation of PKR using 2-aminopurine did not alter reovirus sensitivity in resistant cell lines. Caspase 3 cleavage was not detected in infected cells and oncolysis was observed in pan-caspase inhibited cells.Conclusions: In summary, reovirus is potently oncolytic in a broad panel of SCCHN cell lines. Attempts to define sensitivity/resistance by analysis of the EGFR/Ras/MAPK pathway have failed to provide a clear predictive biomarker of response. Further analysis of material from in vitro and clinical studies is ongoing in an attempt to shed further light on this issue.

Original languageEnglish (US)
Article number368
JournalBMC Cancer
Volume12
DOIs
StatePublished - Aug 24 2012

Fingerprint

Head and Neck Neoplasms
Cell Line
2-Aminopurine
Caspase 3
Biomarkers
Western Blotting
Mitogen-Activated Protein Kinase Kinases
Growth
Caspases
Virion
Inhibitory Concentration 50
Cell Survival
Carcinoma, squamous cell of head and neck
Viruses
Neoplasms

Keywords

  • Biomarker
  • Cancer
  • EGFR
  • Oncolytic virus
  • Ras
  • Reovirus

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Genetics

Cite this

Twigger, K., Roulstone, V., Kyula, J., Karapanagiotou, E. M., Syrigos, K. N., Morgan, R., ... Harrington, K. J. (2012). Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway. BMC Cancer, 12, [368]. https://doi.org/10.1186/1471-2407-12-368

Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway. / Twigger, Katie; Roulstone, Victoria; Kyula, Joan; Karapanagiotou, Eleni M.; Syrigos, Konstantinos N.; Morgan, Richard; White, Christine; Bhide, Shreerang; Nuovo, Gerard; Coffey, Matt; Thompson, Brad; Jebar, Adel; Errington, Fiona; Melcher, Alan A.; Vile, Richard Geoffrey; Pandha, Hardev S.; Harrington, Kevin J.

In: BMC Cancer, Vol. 12, 368, 24.08.2012.

Research output: Contribution to journalArticle

Twigger, K, Roulstone, V, Kyula, J, Karapanagiotou, EM, Syrigos, KN, Morgan, R, White, C, Bhide, S, Nuovo, G, Coffey, M, Thompson, B, Jebar, A, Errington, F, Melcher, AA, Vile, RG, Pandha, HS & Harrington, KJ 2012, 'Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway', BMC Cancer, vol. 12, 368. https://doi.org/10.1186/1471-2407-12-368
Twigger, Katie ; Roulstone, Victoria ; Kyula, Joan ; Karapanagiotou, Eleni M. ; Syrigos, Konstantinos N. ; Morgan, Richard ; White, Christine ; Bhide, Shreerang ; Nuovo, Gerard ; Coffey, Matt ; Thompson, Brad ; Jebar, Adel ; Errington, Fiona ; Melcher, Alan A. ; Vile, Richard Geoffrey ; Pandha, Hardev S. ; Harrington, Kevin J. / Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway. In: BMC Cancer. 2012 ; Vol. 12.
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title = "Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway",
abstract = "Background: Reovirus exploits aberrant signalling downstream of Ras to mediate tumor-specific oncolysis. Since ~90{\%} squamous cell carcinomas of the head and neck (SCCHN) over-express EGFR and SCCHN cell lines are sensitive to oncolytic reovirus, we conducted a detailed analysis of the effects of reovirus in 15 head and neck cancer cell lines. Both pre- and post-entry events were studied in an attempt to define biomarkers predictive of sensitivity/resistance to reovirus. In particular, we analysed the role of EGFR/Ras signalling in determining virus-mediated cytotoxicity in SCCHN.Methods: To test whether EGFR pathway activity was predictive of increased sensitivity to reovirus, correlative analyses between reoviral IC50 by MTT assay and EGFR levels by western blot and FACS were conducted. Inhibition or stimulation of EGFR signalling were analysed for their effect on reoviral oncolysis by MTT assay, and viral growth by TCID50 assay. We next analysed the effects of inhibiting signalling downstream of Ras, by specific inhibitors of p38MAPK, PI3-K or MEK, on reoviral killing examined by MTT assay. The role of PKR in reoviral killing was also determined by blockade of PKR using 2-aminopurine and assaying for cell survival by MTT assay. The apoptotic response of SCCHN to reovirus was examined by western blot analysis of caspase 3 cleavage.Results: Correlative analyses between reoviral sensitivity and EGFR levels revealed no association. Intermediate sub-viral and core particles showed the same infectivity/cytotoxicity as intact reovirus. Therefore, sensitivity was not determined by cell entry. In 4 cell lines, oncolysis and viral growth were both unaffected by inhibition or stimulation of EGFR signalling. Inhibition of signalling downstream of Ras did not abrogate reoviral oncolysis and, in addition, modulation of PKR using 2-aminopurine did not alter reovirus sensitivity in resistant cell lines. Caspase 3 cleavage was not detected in infected cells and oncolysis was observed in pan-caspase inhibited cells.Conclusions: In summary, reovirus is potently oncolytic in a broad panel of SCCHN cell lines. Attempts to define sensitivity/resistance by analysis of the EGFR/Ras/MAPK pathway have failed to provide a clear predictive biomarker of response. Further analysis of material from in vitro and clinical studies is ongoing in an attempt to shed further light on this issue.",
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author = "Katie Twigger and Victoria Roulstone and Joan Kyula and Karapanagiotou, {Eleni M.} and Syrigos, {Konstantinos N.} and Richard Morgan and Christine White and Shreerang Bhide and Gerard Nuovo and Matt Coffey and Brad Thompson and Adel Jebar and Fiona Errington and Melcher, {Alan A.} and Vile, {Richard Geoffrey} and Pandha, {Hardev S.} and Harrington, {Kevin J.}",
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TY - JOUR

T1 - Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway

AU - Twigger, Katie

AU - Roulstone, Victoria

AU - Kyula, Joan

AU - Karapanagiotou, Eleni M.

AU - Syrigos, Konstantinos N.

AU - Morgan, Richard

AU - White, Christine

AU - Bhide, Shreerang

AU - Nuovo, Gerard

AU - Coffey, Matt

AU - Thompson, Brad

AU - Jebar, Adel

AU - Errington, Fiona

AU - Melcher, Alan A.

AU - Vile, Richard Geoffrey

AU - Pandha, Hardev S.

AU - Harrington, Kevin J.

PY - 2012/8/24

Y1 - 2012/8/24

N2 - Background: Reovirus exploits aberrant signalling downstream of Ras to mediate tumor-specific oncolysis. Since ~90% squamous cell carcinomas of the head and neck (SCCHN) over-express EGFR and SCCHN cell lines are sensitive to oncolytic reovirus, we conducted a detailed analysis of the effects of reovirus in 15 head and neck cancer cell lines. Both pre- and post-entry events were studied in an attempt to define biomarkers predictive of sensitivity/resistance to reovirus. In particular, we analysed the role of EGFR/Ras signalling in determining virus-mediated cytotoxicity in SCCHN.Methods: To test whether EGFR pathway activity was predictive of increased sensitivity to reovirus, correlative analyses between reoviral IC50 by MTT assay and EGFR levels by western blot and FACS were conducted. Inhibition or stimulation of EGFR signalling were analysed for their effect on reoviral oncolysis by MTT assay, and viral growth by TCID50 assay. We next analysed the effects of inhibiting signalling downstream of Ras, by specific inhibitors of p38MAPK, PI3-K or MEK, on reoviral killing examined by MTT assay. The role of PKR in reoviral killing was also determined by blockade of PKR using 2-aminopurine and assaying for cell survival by MTT assay. The apoptotic response of SCCHN to reovirus was examined by western blot analysis of caspase 3 cleavage.Results: Correlative analyses between reoviral sensitivity and EGFR levels revealed no association. Intermediate sub-viral and core particles showed the same infectivity/cytotoxicity as intact reovirus. Therefore, sensitivity was not determined by cell entry. In 4 cell lines, oncolysis and viral growth were both unaffected by inhibition or stimulation of EGFR signalling. Inhibition of signalling downstream of Ras did not abrogate reoviral oncolysis and, in addition, modulation of PKR using 2-aminopurine did not alter reovirus sensitivity in resistant cell lines. Caspase 3 cleavage was not detected in infected cells and oncolysis was observed in pan-caspase inhibited cells.Conclusions: In summary, reovirus is potently oncolytic in a broad panel of SCCHN cell lines. Attempts to define sensitivity/resistance by analysis of the EGFR/Ras/MAPK pathway have failed to provide a clear predictive biomarker of response. Further analysis of material from in vitro and clinical studies is ongoing in an attempt to shed further light on this issue.

AB - Background: Reovirus exploits aberrant signalling downstream of Ras to mediate tumor-specific oncolysis. Since ~90% squamous cell carcinomas of the head and neck (SCCHN) over-express EGFR and SCCHN cell lines are sensitive to oncolytic reovirus, we conducted a detailed analysis of the effects of reovirus in 15 head and neck cancer cell lines. Both pre- and post-entry events were studied in an attempt to define biomarkers predictive of sensitivity/resistance to reovirus. In particular, we analysed the role of EGFR/Ras signalling in determining virus-mediated cytotoxicity in SCCHN.Methods: To test whether EGFR pathway activity was predictive of increased sensitivity to reovirus, correlative analyses between reoviral IC50 by MTT assay and EGFR levels by western blot and FACS were conducted. Inhibition or stimulation of EGFR signalling were analysed for their effect on reoviral oncolysis by MTT assay, and viral growth by TCID50 assay. We next analysed the effects of inhibiting signalling downstream of Ras, by specific inhibitors of p38MAPK, PI3-K or MEK, on reoviral killing examined by MTT assay. The role of PKR in reoviral killing was also determined by blockade of PKR using 2-aminopurine and assaying for cell survival by MTT assay. The apoptotic response of SCCHN to reovirus was examined by western blot analysis of caspase 3 cleavage.Results: Correlative analyses between reoviral sensitivity and EGFR levels revealed no association. Intermediate sub-viral and core particles showed the same infectivity/cytotoxicity as intact reovirus. Therefore, sensitivity was not determined by cell entry. In 4 cell lines, oncolysis and viral growth were both unaffected by inhibition or stimulation of EGFR signalling. Inhibition of signalling downstream of Ras did not abrogate reoviral oncolysis and, in addition, modulation of PKR using 2-aminopurine did not alter reovirus sensitivity in resistant cell lines. Caspase 3 cleavage was not detected in infected cells and oncolysis was observed in pan-caspase inhibited cells.Conclusions: In summary, reovirus is potently oncolytic in a broad panel of SCCHN cell lines. Attempts to define sensitivity/resistance by analysis of the EGFR/Ras/MAPK pathway have failed to provide a clear predictive biomarker of response. Further analysis of material from in vitro and clinical studies is ongoing in an attempt to shed further light on this issue.

KW - Biomarker

KW - Cancer

KW - EGFR

KW - Oncolytic virus

KW - Ras

KW - Reovirus

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U2 - 10.1186/1471-2407-12-368

DO - 10.1186/1471-2407-12-368

M3 - Article

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AN - SCOPUS:84865287313

VL - 12

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

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