Renal localization and actions of adrenomedullin: A natriuretic peptide

M. Jougasaki, C. M. Wei, L. L. Aarhus, D. M. Heublein, S. M. Sandberg, J. C. Burnett

Research output: Contribution to journalArticle

251 Scopus citations

Abstract

Adrenomedullin (ADM) is a newly described 52-amino acid peptide originally isolated from extracts of human pheochromocytoma and, more recently, detected in human plasma. Based on the report that ADM mRNA and immunoreactivity are present in the kidney, the current study was designed to determine the renal distribution of ADM by immunohistochemistry and the renal biological actions of ADM. In the immunohistochemical studies, the present investigation demonstrated the localization of ADM in glomeruli, cortical distal tubules, and medullary collecting duct cells of the normal canine kidney. In the in vivo studies, ADM was administered (0.25 ng · kg-1 · min-1 in group I and 1, 5, and 25 ng · kg-1 · min-1 in group II) intrarenally in normal mongrel dogs with the contralateral kidney receiving only saline vehicle. Intrarenal infusion of ADM resulted in a marked diuretic and natriuretic response, whereas the contrallateral kidney showed no renal effects. These significant natriuresis and diuresis in the ADM kidney were associated with increases in glomerular filtration rate and fractional sodium excretion and with a decrease in distal tubular sodium reabsorption. Intrarenal infusion of ADM also caused an increase in mean arterial blood pressure and a decrease in heart rate. Plasma concentrations of atrial natriuretic peptide, renin activity, aldosterone, and guanosine 3',5'-cyclic monophosphate were not changed during the infusion of ADM. The current study demonstrates that ADM is present in renal glomerular and tubular cells and is a potent natriuretic peptide that may play an important role in the regulation of sodium excretion.

Original languageEnglish (US)
Pages (from-to)F657-F663
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume268
Issue number4 37-4
DOIs
StatePublished - 1995

Keywords

  • distal tubular function
  • guanosine 3',5'-cyclic monophosphate
  • immunohistochemistry
  • natriuretic peptide
  • sodium excretion

ASJC Scopus subject areas

  • Physiology

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