Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals

John C Lieske, Hewson Swift, Terence Martin, Briony Patterson, F. Gary Toback

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Renal tubular fluid is supersaturated with calcium and oxalate ions, which can nucleate to form crystals of calcium oxalate monohydrate (COM), the most abundant constituent of kidney stones. However, the mechanisms by which nascent crystals are retained in the nephron and then grow into kidney stones are unclear. An interaction of COM crystals with the surface of renal epithelial cells could be a critical initiating event in nephrolithiasis. To investigate this possibility we used cultures of monkey kidney epithelial cells (BSC-1 line) as a model system and found that [14C]COM crystals bound to the cell surface within seconds. Scanning electron microscopy revealed that crystals bind first to apical microvilli, which subsequently migrate over the crystalline surface. When visualized by transmission electron microscopy, intracellular crystals were located within vesicles. Cytoskeletal responses to crystal uptake were sought by immunofluorescence microscopy, which revealed concentration of F-actin at sites of crystal contact as well as a generalized reorganization of the intermediate filament network containing cytokeratin 8. Uptake of COM crystals did not adversely affect renal epithelial cell growth, and internalized crystals were apparently distributed to daughter cells during division. Rapid adherence of COM crystals to the apical surface of tubular epithelial cells could promote crystal retention in the kidney. Elucidation of factors that regulate this process may provide insight into the pathogenesis of nephrolithiasis.

Original languageEnglish (US)
Pages (from-to)6987-6991
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number15
DOIs
StatePublished - Jul 19 1994
Externally publishedYes

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Calcium Oxalate
Epithelial Cells
Kidney
Nephrolithiasis
Kidney Calculi
Keratin-8
Intermediate Filaments
Nephrons
Microvilli
Crystallization
Transmission Electron Microscopy
Fluorescence Microscopy
Cell Division
Electron Scanning Microscopy
Haplorhini
Actins
Ions

Keywords

  • actin
  • cytoskeleton
  • endocytosis
  • nephrolithiasis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals. / Lieske, John C; Swift, Hewson; Martin, Terence; Patterson, Briony; Toback, F. Gary.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 15, 19.07.1994, p. 6987-6991.

Research output: Contribution to journalArticle

Lieske, John C ; Swift, Hewson ; Martin, Terence ; Patterson, Briony ; Toback, F. Gary. / Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 15. pp. 6987-6991.
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