Renal cell adaptation to oxalate

Eddie L. Greene, Gerard Farell, Shihui Yu, Tori Matthews, Vivek Kumar, John C Lieske

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Renal manifestations of chronic hyperoxaluria include nephrolithiasis and, when extreme, interstitial scarring and progressive loss of function. Exposure of cultured renal cells to oxalate has been reported to cause cell death, as well as proliferation. The current study was performed to assess the time course and cell-type specificity of these responses. Proximal (LLC-PK1) and distal [cIMCD and primary human renal (HRC1)] renal epithelial cells, as well as interstitial KNRK cells, were exposed to oxalate (0.5-2.0 mM) for 24-72 h. The generation of reactive oxygen species (ROS) was measured using the fluorescent probe DCF, and cell number was determined with CyQuant reagent. HSP-70 expression was assessed via real time PCR and quantitative Western blot. In response to all oxalate concentrations (0.5-2.0 mM) and lengths of exposure (15 min - 2 h), cultured proximal and distal renal epithelial cells and renal fibroblasts generated ROS. After 24 h, cells demonstrated initial cell death and decrease in cell numbers, but by 48-72 h adapted and grew, despite the continued presence of oxalate. This response was associated with increased expression of HSP-70 mRNA and protein. Renal cells in vivo may possess adaptive mechanisms to withstand chronic hyperoxaluria, including increased expression of chaperone molecules such as HSP-70.

Original languageEnglish (US)
Pages (from-to)340-348
Number of pages9
JournalUrological Research
Volume33
Issue number5
DOIs
StatePublished - Nov 2005

Fingerprint

Oxalates
Kidney
Hyperoxaluria
Reactive Oxygen Species
Cell Death
Cell Count
Epithelial Cells
Nephrolithiasis
Fluorescent Dyes
Cicatrix
Real-Time Polymerase Chain Reaction
Cause of Death
Cultured Cells
Fibroblasts
Western Blotting
Cell Proliferation
Messenger RNA

Keywords

  • cIMCD
  • HSP-70
  • KNRK
  • LLC-PK
  • Primary cultures

ASJC Scopus subject areas

  • Urology

Cite this

Greene, E. L., Farell, G., Yu, S., Matthews, T., Kumar, V., & Lieske, J. C. (2005). Renal cell adaptation to oxalate. Urological Research, 33(5), 340-348. https://doi.org/10.1007/s00240-005-0491-5

Renal cell adaptation to oxalate. / Greene, Eddie L.; Farell, Gerard; Yu, Shihui; Matthews, Tori; Kumar, Vivek; Lieske, John C.

In: Urological Research, Vol. 33, No. 5, 11.2005, p. 340-348.

Research output: Contribution to journalArticle

Greene, EL, Farell, G, Yu, S, Matthews, T, Kumar, V & Lieske, JC 2005, 'Renal cell adaptation to oxalate', Urological Research, vol. 33, no. 5, pp. 340-348. https://doi.org/10.1007/s00240-005-0491-5
Greene EL, Farell G, Yu S, Matthews T, Kumar V, Lieske JC. Renal cell adaptation to oxalate. Urological Research. 2005 Nov;33(5):340-348. https://doi.org/10.1007/s00240-005-0491-5
Greene, Eddie L. ; Farell, Gerard ; Yu, Shihui ; Matthews, Tori ; Kumar, Vivek ; Lieske, John C. / Renal cell adaptation to oxalate. In: Urological Research. 2005 ; Vol. 33, No. 5. pp. 340-348.
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