TY - JOUR
T1 - Remyelination-promoting antibodies activate distinct Ca2+ influx pathways in astrocytes and oligodendrocytes
T2 - Relationship to the mechanism of myelin repair
AU - Soldán, M. Mateo Paz
AU - Warrington, Arthur E.
AU - Bieber, Allan J.
AU - Ciric, Bogoljub
AU - Van Keulen, Virginia
AU - Pease, Larry R.
AU - Rodriguez, Moses
N1 - Funding Information:
We thank the generosity of Mr. and Mrs. E. Applebaum. This work was supported by National Institutes of Health Grants NS24180 and NS11006 and by Acorda Therapeutics.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Our laboratory has identified mouse and human monoclonal antibodies that promote myelin repair in multiple models of demyelinating disease. We have proposed that these antibodies promote remyelination by directly activating central nervous system glia. Intracellular calcium concentration was monitored using a Fura2 ratiometric assay. Repair-promoting antibodies induced distinct Ca2+ signals in both astrocytes and oligodendrocytes. Astrocyte Ca2+ signaling is mediated by a phospholipase C-dependent pathway while oligodendrocyte Ca2+ signaling is mediated via AMPA-sensitive glutamate receptors. An antibody's ability to induce Ca2+ signals is statistically correlated with promotion of myelin repair. These findings support the hypothesis that remyelination-promoting antibodies are acting directly at the surface of glial cells to induce calcium-dependent physiologic reparative function.
AB - Our laboratory has identified mouse and human monoclonal antibodies that promote myelin repair in multiple models of demyelinating disease. We have proposed that these antibodies promote remyelination by directly activating central nervous system glia. Intracellular calcium concentration was monitored using a Fura2 ratiometric assay. Repair-promoting antibodies induced distinct Ca2+ signals in both astrocytes and oligodendrocytes. Astrocyte Ca2+ signaling is mediated by a phospholipase C-dependent pathway while oligodendrocyte Ca2+ signaling is mediated via AMPA-sensitive glutamate receptors. An antibody's ability to induce Ca2+ signals is statistically correlated with promotion of myelin repair. These findings support the hypothesis that remyelination-promoting antibodies are acting directly at the surface of glial cells to induce calcium-dependent physiologic reparative function.
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U2 - 10.1016/S1044-7431(02)00018-0
DO - 10.1016/S1044-7431(02)00018-0
M3 - Article
C2 - 12595235
AN - SCOPUS:0037285507
SN - 1044-7431
VL - 22
SP - 14
EP - 24
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -