Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis

Tamas Ordog, Ichiro Takayama, Wai Kwok T Cheung, Sean M. Ward, Kenton M. Sanders

Research output: Contribution to journalArticle

256 Citations (Scopus)

Abstract

Patients with long-standing diabetes commonly suffer from gastric neuromuscular dysfunction (gastropathy) causing symptoms ranging from postprandial bloating to recurrent vomiting. Autonomic neuropathy is generally believed to be responsible for diabetic gastropathy and the underlying impairments in gastric emptying (gastroparesis) and receptive relaxation, but the specific mechanisms have not been elucidated. Recently, it has been recognized that interstitial cells of Cajal generate electrical pacemaker activity and mediate motor neurotransmission in the stomach. Loss or defects in interstitial cells could contribute to the development of diabetic gastroparesis. Gastric motility was characterized in spontaneously diabetic NOD/LtJ mice by measuring gastric emptying and by monitoring spontaneous and induced electrical activity in circular smooth muscle cells. Interstitial cells of Cajal were studied by Kit immunofluorescence and transmission electron microscopy. Diabetic mice developed delayed gastric emptying, impaired electrical pacemaking, and reduced motor neurotransmission. Interstitial cells of Cajal were greatly reduced in the distal stomach, and the normally close associations between these cells and enteric nerve terminals were infrequent. Our observations suggest that damage to interstitial cells of Cajal may play a key role in the pathogenesis of diabetic gastropathy.

Original languageEnglish (US)
Pages (from-to)1731-1739
Number of pages9
JournalDiabetes
Volume49
Issue number10
StatePublished - 2000
Externally publishedYes

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Interstitial Cells of Cajal
Gastroparesis
Gastric Emptying
Stomach
Synaptic Transmission
Inbred NOD Mouse
Transmission Electron Microscopy
Smooth Muscle Myocytes
Vomiting
Fluorescent Antibody Technique
Motor Activity
Neurons

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Ordog, T., Takayama, I., Cheung, W. K. T., Ward, S. M., & Sanders, K. M. (2000). Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis. Diabetes, 49(10), 1731-1739.

Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis. / Ordog, Tamas; Takayama, Ichiro; Cheung, Wai Kwok T; Ward, Sean M.; Sanders, Kenton M.

In: Diabetes, Vol. 49, No. 10, 2000, p. 1731-1739.

Research output: Contribution to journalArticle

Ordog, T, Takayama, I, Cheung, WKT, Ward, SM & Sanders, KM 2000, 'Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis', Diabetes, vol. 49, no. 10, pp. 1731-1739.
Ordog, Tamas ; Takayama, Ichiro ; Cheung, Wai Kwok T ; Ward, Sean M. ; Sanders, Kenton M. / Remodeling of networks of interstitial cells of Cajal in a murine model of diabetic gastroparesis. In: Diabetes. 2000 ; Vol. 49, No. 10. pp. 1731-1739.
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