Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1

Anna M. Sawka, Ravinder J. Singh, H. Jay Hiddinga, Joseph P. McConnell, Norman L. Eberhardt, Noel M. Caplice, Timothy O'Brien

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Remnant lipoproteins (RLPs) accumulate in type III hyperlipoproteinemia, a condition associated with significant cardiovascular morbidity. The effect of RLPs on fibrinolysis is unknown. Our aim was to study the effect of RLPs on endothelial expression of plasminogen activator inhibitor-1 (PAI-1). After 24-h culture of human aortic endothelial cells with RLPs at concentrations of 0 (control), 0.038, or 0.076 mg triglyceride/ mL, postculture PAI-1 antigen concentrations were: 870 ± 80, 1963 ± 183 (P = 0.005), and 3551 ± 177 ng/mL (P < 0.001), respectively. Furthermore, after 24-h incubation of endothelial cells with RLPs (0 or 0.076 mg triglyceride/mL), PAI-1 activity increased from 0.667 ± 0.144 to 1.268 ± 0.198 U/mL, respectively (P = 0.008) and endothelial PAI-1 mRNA increased to 2.7 ± 0.66 that of control (P = 0.048). In conclusion, RLPs from patients with type III hyperlipoproteinemia induce endothelial cell PAI-1 expression, which may contribute to a prothrombotic state.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume285
Issue number1
DOIs
StatePublished - Jan 1 2001

Keywords

  • Endothelial cells
  • Fibrinolysis
  • Plasminogen activator inhibitor-1
  • Remnant lipoproteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Sawka, A. M., Singh, R. J., Hiddinga, H. J., McConnell, J. P., Eberhardt, N. L., Caplice, N. M., & O'Brien, T. (2001). Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1. Biochemical and Biophysical Research Communications, 285(1), 15-19. https://doi.org/10.1006/bbrc.2001.5117