Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1

Anna M. Sawka, Ravinder Jit Singh, H. Jay Hiddinga, Joseph P. McConnell, Norman L. Eberhardt, Noel M. Caplice, Timothy O'Brien

Research output: Contribution to journalArticle

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Abstract

Remnant lipoproteins (RLPs) accumulate in type III hyperlipoproteinemia, a condition associated with significant cardiovascular morbidity. The effect of RLPs on fibrinolysis is unknown. Our aim was to study the effect of RLPs on endothelial expression of plasminogen activator inhibitor-1 (PAI-1). After 24-h culture of human aortic endothelial cells with RLPs at concentrations of 0 (control), 0.038, or 0.076 mg triglyceride/ mL, postculture PAI-1 antigen concentrations were: 870 ± 80, 1963 ± 183 (P = 0.005), and 3551 ± 177 ng/mL (P < 0.001), respectively. Furthermore, after 24-h incubation of endothelial cells with RLPs (0 or 0.076 mg triglyceride/mL), PAI-1 activity increased from 0.667 ± 0.144 to 1.268 ± 0.198 U/mL, respectively (P = 0.008) and endothelial PAI-1 mRNA increased to 2.7 ± 0.66 that of control (P = 0.048). In conclusion, RLPs from patients with type III hyperlipoproteinemia induce endothelial cell PAI-1 expression, which may contribute to a prothrombotic state.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume285
Issue number1
DOIs
StatePublished - 2001

Fingerprint

Plasminogen Inactivators
Plasminogen Activator Inhibitor 1
Lipoproteins
Endothelial cells
Hyperlipoproteinemia Type III
Endothelial Cells
Triglycerides
Fibrinolysis
Cell culture
Morbidity
Antigens
Messenger RNA

Keywords

  • Endothelial cells
  • Fibrinolysis
  • Plasminogen activator inhibitor-1
  • Remnant lipoproteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sawka, A. M., Singh, R. J., Hiddinga, H. J., McConnell, J. P., Eberhardt, N. L., Caplice, N. M., & O'Brien, T. (2001). Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1. Biochemical and Biophysical Research Communications, 285(1), 15-19. https://doi.org/10.1006/bbrc.2001.5117

Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1. / Sawka, Anna M.; Singh, Ravinder Jit; Hiddinga, H. Jay; McConnell, Joseph P.; Eberhardt, Norman L.; Caplice, Noel M.; O'Brien, Timothy.

In: Biochemical and Biophysical Research Communications, Vol. 285, No. 1, 2001, p. 15-19.

Research output: Contribution to journalArticle

Sawka, AM, Singh, RJ, Hiddinga, HJ, McConnell, JP, Eberhardt, NL, Caplice, NM & O'Brien, T 2001, 'Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1', Biochemical and Biophysical Research Communications, vol. 285, no. 1, pp. 15-19. https://doi.org/10.1006/bbrc.2001.5117
Sawka, Anna M. ; Singh, Ravinder Jit ; Hiddinga, H. Jay ; McConnell, Joseph P. ; Eberhardt, Norman L. ; Caplice, Noel M. ; O'Brien, Timothy. / Remnant lipoproteins induce endothelial plasminogen activator inhibitor-1. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 285, No. 1. pp. 15-19.
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