Relief of triple-helix-mediated promoter inhibition by elongating RNA polymerases

J. U. Skoog, L James Maher III

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter activity is restored when the triple-helical complexes are disrupted by transcription of either strand of the homopurine operator sequence. The degree of relief from inhibition is related to the frequency of operator transcription. These observations demonstrate that this artificial repressor-operator complex is subject to antagonism by cis elements (other promoters) acting at a distance. Such antagonism might also arise between certain natural transcriptional control regions. Our results suggest that the efficiency of artificial repressors based on triple-helix formation may be limited by transcriptional activity in the gene control region.

Original languageEnglish (US)
Pages (from-to)4055-4058
Number of pages4
JournalNucleic Acids Research
Volume21
Issue number17
StatePublished - 1993
Externally publishedYes

Fingerprint

DNA-Directed RNA Polymerases
Transcription
Helix
RNA
Promoter
Oligonucleotides
Bacteriophages
Antagonism
Operator
Genes
Gene
Triple helix
Demonstrate

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Health, Toxicology and Mutagenesis
  • Applied Mathematics
  • Genetics(clinical)
  • Toxicology

Cite this

Relief of triple-helix-mediated promoter inhibition by elongating RNA polymerases. / Skoog, J. U.; Maher III, L James.

In: Nucleic Acids Research, Vol. 21, No. 17, 1993, p. 4055-4058.

Research output: Contribution to journalArticle

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