Reliability of estimates of pulsatile characteristics of luteinizing hormone and growth hormone release in women

J. Y. Weltman, Johannes D Veldhuis, A. Weltman, J. R. Kerrigan, W. S. Evans, A. D. Rogol

Research output: Contribution to journalArticle

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Abstract

The test-retest reliability of estimates of pulsatile LH and GH release was evaluated in 23 eumenorrheic women during the early follicular phase of the menstrual cycle. Each subject was studied during two successive or near-successive menstrual cycles by repetitive blood sampling every 10 min for 24 h. Pulsatile parameters for LH and GH release were identified and characterized using the Cluster pulse detection algorithm. For LH, no significant differences existed in any parameter mean between the two 24-h admissions. Correlation coefficients for consecutive 24-h studies ranged from r = 0.22 (P < 0.32) for number of LH peaks to r = 0.79 (P < 0.0001) for 24-h integrated LH values (area under the concentration vs. time curve). No significant mean differences in any parameter were observed for consecutive 24-h GH evaluations. Correlation coefficients for 24-h GH ranged from r = 0.25 (P < 0.34) for nadir to r = 0.71 (P < 0.002) for incremental peak increase. Cosinor analysis was used to determine significant 24-h variations in LH and GH concentrations. Statistically significant differences existed between admissions for the amplitude of the nyctohemeral LH rhythm and its acrophase (time at which maximal hormone value was attained), but no mean differences were found for mesor (mean concentration). Correlation coefficients for LH were r = 0.10 (P < 0.65), r = 0.43 (P < 0.08), and r = 0.78 (P < 0.0001) for phase, amplitude, and mesor, respectively. No significant mean differences existed for any parameter of nyctohemeral GH rhythms. Correlation coefficients were r = -0.18 (P < 0.52), r = 0.49 (P < 0.72), and r = 0.14 (P < 0.80) for 24-h GH amplitude, phase, and mesor, respectively. We conclude that comparisons of mean and integrated LH and GH concentrations over a 24-h interval in the early follicular phase of the menstrual cycle are reliable; however, certain pulsatile properties responsible for the achievement of the mean daily concentrations of LH and GH may be nonuniform from menstrual cycle to menstrual cycle. In addition, nonuniformities may exist in the nyctohemeral rhythms of serum concentrations of LH and GH in the adult woman between cycles when a single 24-h time series is the basis for the analysis.

Original languageEnglish (US)
Pages (from-to)1646-1652
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume71
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

Menstrual Cycle
Luteinizing Hormone
Circadian Rhythm
Growth Hormone
Time series
Blood
Follicular Phase
Hormones
Sampling
Reproducibility of Results
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Weltman, J. Y., Veldhuis, J. D., Weltman, A., Kerrigan, J. R., Evans, W. S., & Rogol, A. D. (1990). Reliability of estimates of pulsatile characteristics of luteinizing hormone and growth hormone release in women. Journal of Clinical Endocrinology and Metabolism, 71(6), 1646-1652.

Reliability of estimates of pulsatile characteristics of luteinizing hormone and growth hormone release in women. / Weltman, J. Y.; Veldhuis, Johannes D; Weltman, A.; Kerrigan, J. R.; Evans, W. S.; Rogol, A. D.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 71, No. 6, 1990, p. 1646-1652.

Research output: Contribution to journalArticle

Weltman, JY, Veldhuis, JD, Weltman, A, Kerrigan, JR, Evans, WS & Rogol, AD 1990, 'Reliability of estimates of pulsatile characteristics of luteinizing hormone and growth hormone release in women', Journal of Clinical Endocrinology and Metabolism, vol. 71, no. 6, pp. 1646-1652.
Weltman, J. Y. ; Veldhuis, Johannes D ; Weltman, A. ; Kerrigan, J. R. ; Evans, W. S. ; Rogol, A. D. / Reliability of estimates of pulsatile characteristics of luteinizing hormone and growth hormone release in women. In: Journal of Clinical Endocrinology and Metabolism. 1990 ; Vol. 71, No. 6. pp. 1646-1652.
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abstract = "The test-retest reliability of estimates of pulsatile LH and GH release was evaluated in 23 eumenorrheic women during the early follicular phase of the menstrual cycle. Each subject was studied during two successive or near-successive menstrual cycles by repetitive blood sampling every 10 min for 24 h. Pulsatile parameters for LH and GH release were identified and characterized using the Cluster pulse detection algorithm. For LH, no significant differences existed in any parameter mean between the two 24-h admissions. Correlation coefficients for consecutive 24-h studies ranged from r = 0.22 (P < 0.32) for number of LH peaks to r = 0.79 (P < 0.0001) for 24-h integrated LH values (area under the concentration vs. time curve). No significant mean differences in any parameter were observed for consecutive 24-h GH evaluations. Correlation coefficients for 24-h GH ranged from r = 0.25 (P < 0.34) for nadir to r = 0.71 (P < 0.002) for incremental peak increase. Cosinor analysis was used to determine significant 24-h variations in LH and GH concentrations. Statistically significant differences existed between admissions for the amplitude of the nyctohemeral LH rhythm and its acrophase (time at which maximal hormone value was attained), but no mean differences were found for mesor (mean concentration). Correlation coefficients for LH were r = 0.10 (P < 0.65), r = 0.43 (P < 0.08), and r = 0.78 (P < 0.0001) for phase, amplitude, and mesor, respectively. No significant mean differences existed for any parameter of nyctohemeral GH rhythms. Correlation coefficients were r = -0.18 (P < 0.52), r = 0.49 (P < 0.72), and r = 0.14 (P < 0.80) for 24-h GH amplitude, phase, and mesor, respectively. We conclude that comparisons of mean and integrated LH and GH concentrations over a 24-h interval in the early follicular phase of the menstrual cycle are reliable; however, certain pulsatile properties responsible for the achievement of the mean daily concentrations of LH and GH may be nonuniform from menstrual cycle to menstrual cycle. In addition, nonuniformities may exist in the nyctohemeral rhythms of serum concentrations of LH and GH in the adult woman between cycles when a single 24-h time series is the basis for the analysis.",
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AU - Rogol, A. D.

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N2 - The test-retest reliability of estimates of pulsatile LH and GH release was evaluated in 23 eumenorrheic women during the early follicular phase of the menstrual cycle. Each subject was studied during two successive or near-successive menstrual cycles by repetitive blood sampling every 10 min for 24 h. Pulsatile parameters for LH and GH release were identified and characterized using the Cluster pulse detection algorithm. For LH, no significant differences existed in any parameter mean between the two 24-h admissions. Correlation coefficients for consecutive 24-h studies ranged from r = 0.22 (P < 0.32) for number of LH peaks to r = 0.79 (P < 0.0001) for 24-h integrated LH values (area under the concentration vs. time curve). No significant mean differences in any parameter were observed for consecutive 24-h GH evaluations. Correlation coefficients for 24-h GH ranged from r = 0.25 (P < 0.34) for nadir to r = 0.71 (P < 0.002) for incremental peak increase. Cosinor analysis was used to determine significant 24-h variations in LH and GH concentrations. Statistically significant differences existed between admissions for the amplitude of the nyctohemeral LH rhythm and its acrophase (time at which maximal hormone value was attained), but no mean differences were found for mesor (mean concentration). Correlation coefficients for LH were r = 0.10 (P < 0.65), r = 0.43 (P < 0.08), and r = 0.78 (P < 0.0001) for phase, amplitude, and mesor, respectively. No significant mean differences existed for any parameter of nyctohemeral GH rhythms. Correlation coefficients were r = -0.18 (P < 0.52), r = 0.49 (P < 0.72), and r = 0.14 (P < 0.80) for 24-h GH amplitude, phase, and mesor, respectively. We conclude that comparisons of mean and integrated LH and GH concentrations over a 24-h interval in the early follicular phase of the menstrual cycle are reliable; however, certain pulsatile properties responsible for the achievement of the mean daily concentrations of LH and GH may be nonuniform from menstrual cycle to menstrual cycle. In addition, nonuniformities may exist in the nyctohemeral rhythms of serum concentrations of LH and GH in the adult woman between cycles when a single 24-h time series is the basis for the analysis.

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