Release of neutral proteinases from mononuclear phagocytes and synovial cells in response to cartilaginous wear particles in vitro

Christopher H Evans, Dana C. Mears, James L. Cosgrove

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cartilaginous wear particles were retrieved from synovial fluid aspirates of human diarthrodial joints and added to cultures of human or murine mononuclear phagocytes or human synovial cells. In each case, addition of the wear particles elevated the production of proteinases active at neutral pH against collagen, gelatin, azocasein and the synthetic pentapeptide phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg. Synovial cells secreted more than five times as much collagenase as the same number of the other cells. All types of cell secreted significant quantities of enzymes active against the noncollagenous substrates. Mild treatment of the spent media with trypsin stimulated all of these enzymic activities. The spent culture media of synovial cells which had been exposed to cartilaginous wear particles released hydroxyproline and glycosaminoglycan from powdered cartilage, indicating the production of enzymes which degrade both the collagen and proteoglycan of the cartilaginous matrix. Cultures of mononuclear phagocytes, in contrast, while solubilizing chondroitin sulphate from cartilage, released very little hydroxyproline. The ability of wear particles to elicit these effects suggests a role for them in the pathogenesis of oesteoarthritis and other types of joint deterioration.

Original languageEnglish (US)
Pages (from-to)287-294
Number of pages8
JournalBBA - General Subjects
Volume677
Issue number2
DOIs
StatePublished - Oct 12 1981
Externally publishedYes

Fingerprint

Phagocytes
Peptide Hydrolases
Wear of materials
Hydroxyproline
Cartilage
glycylleucine
Collagen
Joints
Chondroitin Sulfates
Synovial Fluid
Collagenases
Proteoglycans
Enzymes
Gelatin
Glycosaminoglycans
Trypsin
Culture Media
Deterioration
Cell Count
Fluids

Keywords

  • (Cartilage)
  • Arthritis
  • Collagenase
  • Protein release
  • Wear particle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Release of neutral proteinases from mononuclear phagocytes and synovial cells in response to cartilaginous wear particles in vitro. / Evans, Christopher H; Mears, Dana C.; Cosgrove, James L.

In: BBA - General Subjects, Vol. 677, No. 2, 12.10.1981, p. 287-294.

Research output: Contribution to journalArticle

@article{3ae401355b2b4f9b80dd1fd54c8aacae,
title = "Release of neutral proteinases from mononuclear phagocytes and synovial cells in response to cartilaginous wear particles in vitro",
abstract = "Cartilaginous wear particles were retrieved from synovial fluid aspirates of human diarthrodial joints and added to cultures of human or murine mononuclear phagocytes or human synovial cells. In each case, addition of the wear particles elevated the production of proteinases active at neutral pH against collagen, gelatin, azocasein and the synthetic pentapeptide phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg. Synovial cells secreted more than five times as much collagenase as the same number of the other cells. All types of cell secreted significant quantities of enzymes active against the noncollagenous substrates. Mild treatment of the spent media with trypsin stimulated all of these enzymic activities. The spent culture media of synovial cells which had been exposed to cartilaginous wear particles released hydroxyproline and glycosaminoglycan from powdered cartilage, indicating the production of enzymes which degrade both the collagen and proteoglycan of the cartilaginous matrix. Cultures of mononuclear phagocytes, in contrast, while solubilizing chondroitin sulphate from cartilage, released very little hydroxyproline. The ability of wear particles to elicit these effects suggests a role for them in the pathogenesis of oesteoarthritis and other types of joint deterioration.",
keywords = "(Cartilage), Arthritis, Collagenase, Protein release, Wear particle",
author = "Evans, {Christopher H} and Mears, {Dana C.} and Cosgrove, {James L.}",
year = "1981",
month = "10",
day = "12",
doi = "10.1016/0304-4165(81)90098-2",
language = "English (US)",
volume = "677",
pages = "287--294",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Release of neutral proteinases from mononuclear phagocytes and synovial cells in response to cartilaginous wear particles in vitro

AU - Evans, Christopher H

AU - Mears, Dana C.

AU - Cosgrove, James L.

PY - 1981/10/12

Y1 - 1981/10/12

N2 - Cartilaginous wear particles were retrieved from synovial fluid aspirates of human diarthrodial joints and added to cultures of human or murine mononuclear phagocytes or human synovial cells. In each case, addition of the wear particles elevated the production of proteinases active at neutral pH against collagen, gelatin, azocasein and the synthetic pentapeptide phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg. Synovial cells secreted more than five times as much collagenase as the same number of the other cells. All types of cell secreted significant quantities of enzymes active against the noncollagenous substrates. Mild treatment of the spent media with trypsin stimulated all of these enzymic activities. The spent culture media of synovial cells which had been exposed to cartilaginous wear particles released hydroxyproline and glycosaminoglycan from powdered cartilage, indicating the production of enzymes which degrade both the collagen and proteoglycan of the cartilaginous matrix. Cultures of mononuclear phagocytes, in contrast, while solubilizing chondroitin sulphate from cartilage, released very little hydroxyproline. The ability of wear particles to elicit these effects suggests a role for them in the pathogenesis of oesteoarthritis and other types of joint deterioration.

AB - Cartilaginous wear particles were retrieved from synovial fluid aspirates of human diarthrodial joints and added to cultures of human or murine mononuclear phagocytes or human synovial cells. In each case, addition of the wear particles elevated the production of proteinases active at neutral pH against collagen, gelatin, azocasein and the synthetic pentapeptide phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg. Synovial cells secreted more than five times as much collagenase as the same number of the other cells. All types of cell secreted significant quantities of enzymes active against the noncollagenous substrates. Mild treatment of the spent media with trypsin stimulated all of these enzymic activities. The spent culture media of synovial cells which had been exposed to cartilaginous wear particles released hydroxyproline and glycosaminoglycan from powdered cartilage, indicating the production of enzymes which degrade both the collagen and proteoglycan of the cartilaginous matrix. Cultures of mononuclear phagocytes, in contrast, while solubilizing chondroitin sulphate from cartilage, released very little hydroxyproline. The ability of wear particles to elicit these effects suggests a role for them in the pathogenesis of oesteoarthritis and other types of joint deterioration.

KW - (Cartilage)

KW - Arthritis

KW - Collagenase

KW - Protein release

KW - Wear particle

UR - http://www.scopus.com/inward/record.url?scp=0019811199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019811199&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(81)90098-2

DO - 10.1016/0304-4165(81)90098-2

M3 - Article

C2 - 7028135

AN - SCOPUS:0019811199

VL - 677

SP - 287

EP - 294

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 2

ER -