Relative induction of cyclobutane dimers and cytosine photohydrates in DNA irradiated in vitro and in vivo with ultraviolet-C and ultraviolet-B light.

D. L. Mitchell, Jin Jen, J. E. Cleaver

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

SV40 DNA was irradiated in vitro and in vivo with UV-C (240-280 nm) and UV-B (280-320 nm) light, and damaged sites sensitive to digestion with Escherichia coli endonuclease III (endo III) and bacteriophage T4 endonuclease V (endo V) were quantified. The frequency of endo III-sensitive sites (primarily cytosine photohydrates) induced was 1-2% of the frequency of endo V-sensitive sites (cyclobutane dimers) in both purified SV40 DNA and intracellular episomal SV40 DNA. Endo III- and endo V-sensitive sites in DNA were induced in the same relative proportion at both UV-C and UV-B wavelengths. We found no evidence to support earlier inferences that intracellular conditions enhance the formation of cytosine photohydrates or other monobasic forms of DNA damage.

Original languageEnglish (US)
Pages (from-to)741-746
Number of pages6
JournalPhotochemistry and Photobiology
Volume54
Issue number5
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Cyclobutanes
cyclobutane
Cytosine
Ultraviolet Rays
Dimers
induction
Deoxyribonuclease (Pyrimidine Dimer)
deoxyribonucleic acid
dimers
Endonucleases
DNA
bacteriophages
DNA Damage
Digestion
Escherichia
inference
Escherichia coli
proportion
Light
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Relative induction of cyclobutane dimers and cytosine photohydrates in DNA irradiated in vitro and in vivo with ultraviolet-C and ultraviolet-B light. / Mitchell, D. L.; Jen, Jin; Cleaver, J. E.

In: Photochemistry and Photobiology, Vol. 54, No. 5, 11.1991, p. 741-746.

Research output: Contribution to journalArticle

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