Relationship between plasma free fatty acid, intramyocellular triglycerides and long-chain acylcarnitines in resting humans

Jill A. Kanaley, Samyah Shadid, Michael T. Sheehan, ZengKui Guo, Michael Dennis Jensen

Research output: Contribution to journalArticle

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Abstract

We hypothesized that plasma non-esterified fatty acids (NEFA) are trafficked directly to intramyocellular long-chain acylcarnitines (imLCAC) rather than transiting intramyocellular triglycerides (imTG) on the way to resting muscle fatty acid oxidation. Overnight fasted adults (n = 61) received intravenous infusions of [U-13C]palmitate (0400-0830 h) and [U-13C]oleate (0800-1400 h) labelling plasma NEFA, imTG, imLCAC and im-non-esterified FA (imNEFA). Two muscle biopsies (0830 and 1400 h) were performed following 6 h, overlapping, sequential palmitate/oleate tracer infusions. Enrichment of plasma palmitate was ∼15 times greater than enrichment of imTG, imNEFA-palmitate and im-palmitoyl-carnitine. Fatty acid enrichment in LCAC was correlated with imTG and imNEFA; there was a significant correlation between imTG concentrations and imLCAC concentrations in women (r = 0.51, P = 0.005), but not men (r = 0.30, P = 0.11). We estimated that ∼11% of NEFA were stored in imTG. imTG NEFA storage was correlated only with NEFA concentrations (r = 0.52, P = 0.004) in women and with (r = 0.45, P = 0.02) in men. At rest, plasma NEFA are trafficked largely to imTG before they enter LCAC oxidative pools; thus, imTG are an important, central pool that regulates the delivery of fatty acids to the intracellular environment. Factors relating to plasma NEFA storage into imTG differ in men and women.

Original languageEnglish (US)
Pages (from-to)5939-5950
Number of pages12
JournalJournal of Physiology
Volume587
Issue number24
DOIs
StatePublished - Dec 2009

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Nonesterified Fatty Acids
Triglycerides
Fatty Acids
Palmitates
Oleic Acid
acylcarnitine
Muscles
Carnitine
Intravenous Infusions
Biopsy

ASJC Scopus subject areas

  • Physiology

Cite this

Relationship between plasma free fatty acid, intramyocellular triglycerides and long-chain acylcarnitines in resting humans. / Kanaley, Jill A.; Shadid, Samyah; Sheehan, Michael T.; Guo, ZengKui; Jensen, Michael Dennis.

In: Journal of Physiology, Vol. 587, No. 24, 12.2009, p. 5939-5950.

Research output: Contribution to journalArticle

Kanaley, Jill A. ; Shadid, Samyah ; Sheehan, Michael T. ; Guo, ZengKui ; Jensen, Michael Dennis. / Relationship between plasma free fatty acid, intramyocellular triglycerides and long-chain acylcarnitines in resting humans. In: Journal of Physiology. 2009 ; Vol. 587, No. 24. pp. 5939-5950.
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