Abstract
Estimates of T regulatory cell populations in the periphery of patients with Crohn's disease are confounded by disease activity and concomitant immunotherapeutic agents known to affect T cell proliferation and survival. We performed deuterium pulse/chase experiments in patients with quiescent Crohn's disease on no immunotherapy and healthy control subjects to estimate T regulatory cell kinetics. Quantification of deuterated DNA isolated from T cell subsets over 10. days was determined by mass spectrophotometry. We demonstrate enhanced proliferation within the T regulatory cell population from patients with Crohn's disease when compared to non-T regulatory cells and T regulatory cells from healthy control subjects. We speculate that T regulatory cells isolated from the periphery of patients with Crohn's disease experience persistent antigen stimulation resulting in excess proliferative rates.
Original language | English (US) |
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Pages (from-to) | 145-150 |
Number of pages | 6 |
Journal | Human Immunology |
Volume | 74 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2013 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology