Regulatory T cell kinetics in the peripheral blood of patients with Crohn's disease

Meher K. Rahman, Chetan P. Offord, Sahil Khanna, Godfrey C. Ford, Xuan Mai T. Perrson, Phyllis A. Svingen, Yuning Xiong, Zeljko Bajzer, William A. Faubion

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Estimates of T regulatory cell populations in the periphery of patients with Crohn's disease are confounded by disease activity and concomitant immunotherapeutic agents known to affect T cell proliferation and survival. We performed deuterium pulse/chase experiments in patients with quiescent Crohn's disease on no immunotherapy and healthy control subjects to estimate T regulatory cell kinetics. Quantification of deuterated DNA isolated from T cell subsets over 10. days was determined by mass spectrophotometry. We demonstrate enhanced proliferation within the T regulatory cell population from patients with Crohn's disease when compared to non-T regulatory cells and T regulatory cells from healthy control subjects. We speculate that T regulatory cells isolated from the periphery of patients with Crohn's disease experience persistent antigen stimulation resulting in excess proliferative rates.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalHuman Immunology
Volume74
Issue number2
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Rahman, M. K., Offord, C. P., Khanna, S., Ford, G. C., Perrson, X. M. T., Svingen, P. A., Xiong, Y., Bajzer, Z., & Faubion, W. A. (2013). Regulatory T cell kinetics in the peripheral blood of patients with Crohn's disease. Human Immunology, 74(2), 145-150. https://doi.org/10.1016/j.humimm.2012.10.021