Regulatory role of CD8+ T lymphocytes in bone marrow eosinophilopoiesis

Madeleine Rådinger, Svetlana Sergejeva, Anna Karin Johansson, Carina Malmhäll, Apostolos Bossios, Margareta Sjöstrand, James J. Lee, Jan Lötvall

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: There is a growing body of evidence to suggest that CD8+ T lymphocytes contribute to local allergen-induced eosinophilic inflammation. Since bone marrow (BM) responses are intricately involved in the induction of airway eosinophilia, we hypothesized that CD8+ T lymphocytes, as well as CD4+ T lymphocytes, may be involved in this process. Methods: Several approaches were utilized. Firstly, mice over expressing interleukin-5 (IL-5) in CD3+ T lymphocytes (NJ.1638; CD3IL-5+ mice) were bred with gene knockout mice lacking either CD4+ T lymphocytes (CD4-/-) or CD8+ T lymphocytes (CD8-/-) to produce CD3IL-5+ knockout mice deficient in CD4+ T lymphocytes (CD3IL-5+/CD4-/-) and CD8+ T lymphocytes (CD3IL-5+/CD8-/ -), respectively. Secondly, CD3+, CD4+ and CD8+ T lymphocytes from naïve CD3IL-5+ and C57BL/6 mice were adoptively transferred to immunodeficient SCID-bg mice to determine their effect on BM eosinophilia. Thirdly, CD3IL-5+, CD3IL-5+/CD8-/- and CD3IL-5+/CD4-/- mice were sensitized and allergen challenged. Bone marrow and blood samples were collected in all experiments. Results: The number of BM eosinophils was significantly reduced in CD3IL-5+/CD8-/- mice compared to CD3IL-5+ mice and CD3IL-5+/CD4-/- mice. Serum IL-5 was significantly higher in CD3IL-5+/CD4-/- mice compared to CD3IL-5+ mice but there was no difference in serum IL-5 between CD3IL-5+/CD4-/- and CD3IL-5+/CD8-/- mice. Adoptive transfer of CD8+, but not CD4+ T lymphocytes from naïve CD3IL-5+ and C57BL/6 mice restored BM eosinophilia in immunodeficient SCID-bg mice. Additionally, allergen challenged CD3IL-5+/CD8-/- mice developed lower numbers of BM eosinophils compared to CD3IL-5+ mice and CD3IL-5+/CD4-/- mice. Conclusion: This study shows that CD8+ T lymphocytes are intrica tely involved in the regulation of BM eosinophilopoiesis, both in non-sensitized as well as sensitized and allergen challenged mice.

Original languageEnglish (US)
Article number83
JournalRespiratory Research
Volume7
DOIs
StatePublished - Jan 6 2006

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Regulatory role of CD8<sup>+</sup> T lymphocytes in bone marrow eosinophilopoiesis'. Together they form a unique fingerprint.

  • Cite this

    Rådinger, M., Sergejeva, S., Johansson, A. K., Malmhäll, C., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. (2006). Regulatory role of CD8+ T lymphocytes in bone marrow eosinophilopoiesis. Respiratory Research, 7, [83]. https://doi.org/10.1186/1465-9921-7-83