TY - JOUR
T1 - Regulatory properties of the integrated long terminal repeat of the feline immunodeficiency virus
AU - Ikeda, Yasuhiro
AU - Kawaguchi, Yasushi
AU - Tomonaga, Keizo
AU - Inoshima, Yasuo
AU - Kohmoto, Mariko
AU - Miyazawa, Takayuki
AU - Mikami, Takeshi
PY - 1996/4
Y1 - 1996/4
N2 - To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effects of treatment with several chemical agents; infection with feline viruses, or transfection with effector plasmids expressing FIV gene products on FIV LTR-directed gene expression. Among them, treatment with the phorbol ester (a strong activator of protein kinase C), forskolin (an inducer of cyclic-AMP), 5-azacytidine (a DNA methylation antagonist), or infection with feline herpesvirus type 1 (FHV-1), resulted in induction of CAT activity in the cells. These results suggest that the integrated FIV LTR is stimulated by cellular transcriptional factors induced by phorbol ester, forskolin and FHV-1, and is also inactivated by DNA methylation. Furthermore, this permanent cell line can be used as a screening system of activators of the FIV LTR.
AB - To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effects of treatment with several chemical agents; infection with feline viruses, or transfection with effector plasmids expressing FIV gene products on FIV LTR-directed gene expression. Among them, treatment with the phorbol ester (a strong activator of protein kinase C), forskolin (an inducer of cyclic-AMP), 5-azacytidine (a DNA methylation antagonist), or infection with feline herpesvirus type 1 (FHV-1), resulted in induction of CAT activity in the cells. These results suggest that the integrated FIV LTR is stimulated by cellular transcriptional factors induced by phorbol ester, forskolin and FHV-1, and is also inactivated by DNA methylation. Furthermore, this permanent cell line can be used as a screening system of activators of the FIV LTR.
KW - Feline herpesvirus
KW - Long terminal repeat
KW - Phorbol ester
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U2 - 10.1016/0168-1702(96)01284-1
DO - 10.1016/0168-1702(96)01284-1
M3 - Article
C2 - 8738180
AN - SCOPUS:0029952375
SN - 0168-1702
VL - 41
SP - 201
EP - 207
JO - Virus Research
JF - Virus Research
IS - 2
ER -