Regulatory properties of the integrated long terminal repeat of the feline immunodeficiency virus

Yasuhiro Ikeda, Yasushi Kawaguchi, Keizo Tomonaga, Yasuo Inoshima, Mariko Kohmoto, Takayuki Miyazawa, Takeshi Mikami

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effects of treatment with several chemical agents; infection with feline viruses, or transfection with effector plasmids expressing FIV gene products on FIV LTR-directed gene expression. Among them, treatment with the phorbol ester (a strong activator of protein kinase C), forskolin (an inducer of cyclic-AMP), 5-azacytidine (a DNA methylation antagonist), or infection with feline herpesvirus type 1 (FHV-1), resulted in induction of CAT activity in the cells. These results suggest that the integrated FIV LTR is stimulated by cellular transcriptional factors induced by phorbol ester, forskolin and FHV-1, and is also inactivated by DNA methylation. Furthermore, this permanent cell line can be used as a screening system of activators of the FIV LTR.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalVirus Research
Volume41
Issue number2
DOIs
StatePublished - Apr 1996

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Keywords

  • Feline herpesvirus
  • Long terminal repeat
  • Phorbol ester

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Ikeda, Y., Kawaguchi, Y., Tomonaga, K., Inoshima, Y., Kohmoto, M., Miyazawa, T., & Mikami, T. (1996). Regulatory properties of the integrated long terminal repeat of the feline immunodeficiency virus. Virus Research, 41(2), 201-207. https://doi.org/10.1016/0168-1702(96)01284-1