Mouse neuroblastoma (clone NIE 115) cells in the logarithmic growth phase were incubated for 12 days. From early log phase to late stationary phase, the specific activity of tyrosine hydroxylase (EC 1.14.3a) increased by more than 30 fold. The increase in tyrosine hydroxylase per cell and per dish was 12 and 2700 fold, respectively. When cell division was stopped by removing serum or by adding 0.1 mM 5 fluorodeoxyuridine and 0.1 mM uridine, the enzyme activity was also found to increase. These results show that tyrosine hydroxylase is regulated in neuroblastoma clone N1E 115.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Neurochemistry|
|State||Published - 1973|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience