Regulation of the Eβ gene in vivo: Lessons from Eβd transgenic mice

John Douhan, Julia A. Brown, Zachary L. Gleit, Chella S. David, Laurie H. Glimcher

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We have examined the expression and regulation of the MHC class II Eβd gene in both cell lines and in transgenic mice. In transient transfection assays, as little as 192 bp of the Eβd proximal promoter was sufficient to direct constitutive expression of a reporter gene in a B cell line and to confer inducibility by IFN-γ in a macrophage cell line. To determine if the same Eβd promoter sequences were also sufficient to direct correct expression in vivo, Eβd transgenes bearing either 4.1 or 0.2 kb of upstream sequence were introduced into an inbred mouse strain with a nonexpressed endogenous Eβ gene. Expression of both transgenes mirrored the expression of the endogenous I-A protein in thymus, B cells and macrophages with regard to both constitutive and cytokine-inducible expression. These results indicate that for the Eβ gene only 200 bp of proximal promoter sequence are required to achieve tissue-specific and cytokine-inducible expression. This is in striking contrast to the Eα gene, the only other murine class II gene whose promoter has been analyzed in vivo, which has been shown to require 2.0 kb of upstream sequence for appropriate expression. These data demonstrate, therefore, that the location of critical regulatory elements for the Eβ and Eα genes may differ.

Original languageEnglish (US)
Pages (from-to)255-265
Number of pages11
JournalInternational Immunology
Volume8
Issue number2
DOIs
StatePublished - 1996

Keywords

  • Cytokine
  • MHC
  • Regulation
  • Transcription
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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