Regulation of RelB expression during the initiation of dendritic cell differentiation

Pedro J. Cejas, Louise M. Carlson, Despina Kolonias, Jian Zhang, Inna Lindner, Daniel D. Billadeau, Lawrence H. Boise, Kelvin P. Lee

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The transcription factor RelB is required for proper development and function of dendritic cells (DCs), and its expression is upregulated early during differentiation from a variety of progenitors. We explored this mechanism of upregulation in the KG1 cell line model of a DC progenitor and in the differentiation-resistant KG1a subline. RelB expression is relatively higher in untreated KG1a cells but is upregulated only during differentiation of KG1 by an early enhancement of transcriptional elongation, followed by an increase in transcription initiation. Restoration of protein kinase CβII (PKCβII) expression in KG1a cells allows them to differentiate into DCs. We show that PKCβII also downregulated constitutive expression of NF-κB in KG1a-transfected cells and restores the upregulation of RelB during differentiation by increased transcriptional initiation and elongation. The two mechanisms are independent and sensitive to PKC signaling levels. Conversely, RelB upregulation was inhibited in primary human monocytes where PKCβII expression was knocked down by small interfering RNA targeting. Altogether, the data show that RelB expression during DC differentiation is controlled by PKCβII-mediated regulation of transcriptional initiation and elongation.

Original languageEnglish (US)
Pages (from-to)7900-7916
Number of pages17
JournalMolecular and cellular biology
Issue number17
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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