Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide

K. M. Choi, S. J. Gibbons, J. L. Roeder, M. S. Lurken, J. Zhu, M. M. Wouters, S. M. Miller, J. H. Szurszewski, Gianrico Farrugia

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The factors underlying the survival and maintenance of interstitial cells of Cajal (ICC) are not well understood. Loss of ICC is often associated with loss of neuronal nitric oxide synthase (nNOS) in humans, suggesting a possible link. The aim of this study was to determine the effect of neuronal NO on ICC in the mouse gastric body. The volumes of ICC were determined in nNOS -/- and control mice in the gastric body and in organotypic cultures using immunohistochemistry, laser scanning confocal microscopy and three-dimensional reconstruction. ICC numbers were determined in primary cell cultures after treatment with an NO donor or an NOS inhibitor. The volumes of myenteric c-Kit-immunoreactive networks of ICC from nNOS-/- mice were significantly reduced compared with control mice. No significant differences in the volumes of c-Kit-positive ICC were observed in the longitudinal muscle layers. ICC volumes were either decreased or unaltered in the circular muscle layer after normalization for the volume of circular smooth muscle. The number of ICC was increased after incubation with S-nitroso-N-acetylpenicillamine and decreased by N(G)-nitro-l-arginine. Neuronally derived NO modulates ICC numbers and network volume in the mouse gastric body. NO appears to be a survival factor for ICC.

Original languageEnglish (US)
Pages (from-to)585-595
Number of pages11
JournalNeurogastroenterology and Motility
Volume19
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Interstitial Cells of Cajal
Stomach
Nitric Oxide
Nitric Oxide Synthase Type I
Cell Count
S-Nitroso-N-Acetylpenicillamine
Muscles
Primary Cell Culture
Survival
Cell Size
Confocal Microscopy
Smooth Muscle
Arginine

Keywords

  • Gastrointestinal tract
  • Stomach
  • Survival

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this

Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide. / Choi, K. M.; Gibbons, S. J.; Roeder, J. L.; Lurken, M. S.; Zhu, J.; Wouters, M. M.; Miller, S. M.; Szurszewski, J. H.; Farrugia, Gianrico.

In: Neurogastroenterology and Motility, Vol. 19, No. 7, 07.2007, p. 585-595.

Research output: Contribution to journalArticle

Choi, KM, Gibbons, SJ, Roeder, JL, Lurken, MS, Zhu, J, Wouters, MM, Miller, SM, Szurszewski, JH & Farrugia, G 2007, 'Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide', Neurogastroenterology and Motility, vol. 19, no. 7, pp. 585-595. https://doi.org/10.1111/j.1365-2982.2007.00936.x
Choi, K. M. ; Gibbons, S. J. ; Roeder, J. L. ; Lurken, M. S. ; Zhu, J. ; Wouters, M. M. ; Miller, S. M. ; Szurszewski, J. H. ; Farrugia, Gianrico. / Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide. In: Neurogastroenterology and Motility. 2007 ; Vol. 19, No. 7. pp. 585-595.
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