Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells

Sonja L. Toutenhoofd, Emanuel E. Strehler

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Calmodulin (CaM), the principal mediator of the calcium signal, regulates numerous processes pertinent to neural function. Mammalian CaM is generated from three genes that give rise to five distinct transcripts. To determine the regulation of individual CaM transcripts in neurons, we assessed their abundance during differentiation of human IMR-32 neuroblastoma cells. Northern analysis revealed that the 4.1 kb CALM1 transcript was specifically upregulated about two-fold during differentiation, and that this increase correlated with neurite extension. By contrast, the CALM2 and CALM3 mRNAs as well as the 1.7 kb CALM1 transcript showed an initial increase but then returned to levels close to, or only slightly above, controls. The increase in the 4.1 kb transcript was largely due to its specific stabilization in differentiated cells. However, total cellular CaM levels did not change significantly throughout differentiation. To begin to address whether the 4.1 kb CALM1 transcript might play a unique role in providing local CaM pools, we determined its localization in differentiated IMR-32 cells using in situ hybridization. The 4.1 kb CALM1 transcript localized to the cell body, but was also present within extending neurites. This finding agrees with in vivo studies showing elevated levels of the 4.1 kb CALM1 transcript in adult rat central neurons and the presence of CALM1 transcripts in dendrites, and establishes a human in vitro model system to study individual CaM transcripts with respect to neuronal functions.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1600
Issue number1-2
DOIs
StatePublished - Nov 4 2002

Fingerprint

Calmodulin
Neuroblastoma
Messenger RNA
Neurites
Neurons
Dendrites
In Situ Hybridization
Rats
Stabilization
Genes
Cells
Calcium

Keywords

  • Calcium signaling
  • Calmodulin
  • IMR-32 neuroblastoma cell
  • mRNA localization
  • Neuronal differentiation
  • Posttranscriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

Cite this

Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells. / Toutenhoofd, Sonja L.; Strehler, Emanuel E.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1600, No. 1-2, 04.11.2002, p. 95-104.

Research output: Contribution to journalArticle

Toutenhoofd, Sonja L. ; Strehler, Emanuel E. / Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2002 ; Vol. 1600, No. 1-2. pp. 95-104.
@article{44adda4bcd984f698760ce68c04c3e1c,
title = "Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells",
abstract = "Calmodulin (CaM), the principal mediator of the calcium signal, regulates numerous processes pertinent to neural function. Mammalian CaM is generated from three genes that give rise to five distinct transcripts. To determine the regulation of individual CaM transcripts in neurons, we assessed their abundance during differentiation of human IMR-32 neuroblastoma cells. Northern analysis revealed that the 4.1 kb CALM1 transcript was specifically upregulated about two-fold during differentiation, and that this increase correlated with neurite extension. By contrast, the CALM2 and CALM3 mRNAs as well as the 1.7 kb CALM1 transcript showed an initial increase but then returned to levels close to, or only slightly above, controls. The increase in the 4.1 kb transcript was largely due to its specific stabilization in differentiated cells. However, total cellular CaM levels did not change significantly throughout differentiation. To begin to address whether the 4.1 kb CALM1 transcript might play a unique role in providing local CaM pools, we determined its localization in differentiated IMR-32 cells using in situ hybridization. The 4.1 kb CALM1 transcript localized to the cell body, but was also present within extending neurites. This finding agrees with in vivo studies showing elevated levels of the 4.1 kb CALM1 transcript in adult rat central neurons and the presence of CALM1 transcripts in dendrites, and establishes a human in vitro model system to study individual CaM transcripts with respect to neuronal functions.",
keywords = "Calcium signaling, Calmodulin, IMR-32 neuroblastoma cell, mRNA localization, Neuronal differentiation, Posttranscriptional regulation",
author = "Toutenhoofd, {Sonja L.} and Strehler, {Emanuel E.}",
year = "2002",
month = "11",
day = "4",
doi = "10.1016/S1570-9639(02)00449-1",
language = "English (US)",
volume = "1600",
pages = "95--104",
journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",
issn = "1570-9639",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Regulation of calmodulin mRNAs in differentiating human IMR-32 neuroblastoma cells

AU - Toutenhoofd, Sonja L.

AU - Strehler, Emanuel E.

PY - 2002/11/4

Y1 - 2002/11/4

N2 - Calmodulin (CaM), the principal mediator of the calcium signal, regulates numerous processes pertinent to neural function. Mammalian CaM is generated from three genes that give rise to five distinct transcripts. To determine the regulation of individual CaM transcripts in neurons, we assessed their abundance during differentiation of human IMR-32 neuroblastoma cells. Northern analysis revealed that the 4.1 kb CALM1 transcript was specifically upregulated about two-fold during differentiation, and that this increase correlated with neurite extension. By contrast, the CALM2 and CALM3 mRNAs as well as the 1.7 kb CALM1 transcript showed an initial increase but then returned to levels close to, or only slightly above, controls. The increase in the 4.1 kb transcript was largely due to its specific stabilization in differentiated cells. However, total cellular CaM levels did not change significantly throughout differentiation. To begin to address whether the 4.1 kb CALM1 transcript might play a unique role in providing local CaM pools, we determined its localization in differentiated IMR-32 cells using in situ hybridization. The 4.1 kb CALM1 transcript localized to the cell body, but was also present within extending neurites. This finding agrees with in vivo studies showing elevated levels of the 4.1 kb CALM1 transcript in adult rat central neurons and the presence of CALM1 transcripts in dendrites, and establishes a human in vitro model system to study individual CaM transcripts with respect to neuronal functions.

AB - Calmodulin (CaM), the principal mediator of the calcium signal, regulates numerous processes pertinent to neural function. Mammalian CaM is generated from three genes that give rise to five distinct transcripts. To determine the regulation of individual CaM transcripts in neurons, we assessed their abundance during differentiation of human IMR-32 neuroblastoma cells. Northern analysis revealed that the 4.1 kb CALM1 transcript was specifically upregulated about two-fold during differentiation, and that this increase correlated with neurite extension. By contrast, the CALM2 and CALM3 mRNAs as well as the 1.7 kb CALM1 transcript showed an initial increase but then returned to levels close to, or only slightly above, controls. The increase in the 4.1 kb transcript was largely due to its specific stabilization in differentiated cells. However, total cellular CaM levels did not change significantly throughout differentiation. To begin to address whether the 4.1 kb CALM1 transcript might play a unique role in providing local CaM pools, we determined its localization in differentiated IMR-32 cells using in situ hybridization. The 4.1 kb CALM1 transcript localized to the cell body, but was also present within extending neurites. This finding agrees with in vivo studies showing elevated levels of the 4.1 kb CALM1 transcript in adult rat central neurons and the presence of CALM1 transcripts in dendrites, and establishes a human in vitro model system to study individual CaM transcripts with respect to neuronal functions.

KW - Calcium signaling

KW - Calmodulin

KW - IMR-32 neuroblastoma cell

KW - mRNA localization

KW - Neuronal differentiation

KW - Posttranscriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=0037020692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037020692&partnerID=8YFLogxK

U2 - 10.1016/S1570-9639(02)00449-1

DO - 10.1016/S1570-9639(02)00449-1

M3 - Article

C2 - 12445464

AN - SCOPUS:0037020692

VL - 1600

SP - 95

EP - 104

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

SN - 1570-9639

IS - 1-2

ER -