Regulation of biliary secretion through apical purinergic receptors in cultured rat cholangiocytes

Thorsten Schlenker, Joelle M J Romac, Ala I. Sharara, Richard M. Roman, Stephen J. Kim, Nicholas F La Russo, Rodger A. Liddle, J. Gregory Fitz

Research output: Contribution to journalArticle

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Abstract

To evaluate whether ATP in bile serves as a signaling factor regulating ductular secretion, voltage-clamp studies were performed using a novel normal rat cholangiocyte (NRC) model. In the presence of amiloride (100 μM) to block Na+ channels, exposure of the apical membrane to ATP significantly increased the short-circuit current (I(SC)) from 18.2 ± 5.9 to 52.8 ± 12.7 μA (n = 18). The response to ATP is mediated by basolateral-to-apical Cl- transport because it is inhibited by 1) the Cl- channel blockers 4,4'- diisothiocyanostilbene-2,2'-disulfonic acid (1 mM), diphenylanthranilic acid (1.5 mM), or 5-nitro-2-(3-phenylpropylamino)benzoic acid (50 or 100 μM) in the apical chamber, 2) the K+ channel blocker Ba2+ (5 mM), or 3) the Na+- K+-2Cl cotransport inhibitor bumetanide (200 μM) in the basolateral chamber. Other nucleotides stimulated an increase in I(SC) with a rank order potency of UTP = ATP = adenosine 5'-O-(3)-thiotriphosphate, consistent with P(2U) purinergic receptors. ADP, AMP, 2-methylthioadenosine 5'-triphosphate, and adenosine had no effect. A cDNA encoding a rat P(2U) receptor (rP(2U)R) was isolated from a liver cDNA library, and functional expression of the corresponding mRNA in Xenopus laevis oocytes resulted in the appearance of ATP-stimulated currents with a similar pharmacological profile. Northern analysis identified hybridizing mRNA transcripts in NRC as well as other cell types in rat liver. These findings indicate that exposure of polarized cholangiocytes to ATP results in luminal Cl- secretion through activation of P(2U) receptors in the apical membrane. Release of ATP into bile may serve as an autocrine or paracrine signal regulating cholangiocyte secretory function.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume273
Issue number5 36-5
StatePublished - 1997

Fingerprint

Purinergic Receptors
Adenosine Triphosphate
Bile
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Bumetanide
Messenger RNA
Uridine Triphosphate
Membranes
Amiloride
Liver
Xenopus laevis
Adenosine Monophosphate
Gene Library
Adenosine
Adenosine Diphosphate
Oocytes
Nucleotides
Complementary DNA
Pharmacology
Acids

Keywords

  • Bile
  • Chloride channel
  • Ion transport
  • Liver

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Regulation of biliary secretion through apical purinergic receptors in cultured rat cholangiocytes. / Schlenker, Thorsten; Romac, Joelle M J; Sharara, Ala I.; Roman, Richard M.; Kim, Stephen J.; La Russo, Nicholas F; Liddle, Rodger A.; Gregory Fitz, J.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 273, No. 5 36-5, 1997.

Research output: Contribution to journalArticle

Schlenker, Thorsten ; Romac, Joelle M J ; Sharara, Ala I. ; Roman, Richard M. ; Kim, Stephen J. ; La Russo, Nicholas F ; Liddle, Rodger A. ; Gregory Fitz, J. / Regulation of biliary secretion through apical purinergic receptors in cultured rat cholangiocytes. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 ; Vol. 273, No. 5 36-5.
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AU - La Russo, Nicholas F

AU - Liddle, Rodger A.

AU - Gregory Fitz, J.

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