Regulation of anterior chamber drainage by bicarbonatesensitive soluble adenylyl cyclase in the ciliary body

Yong S. Lee, Martin Tresguerres, Kenneth Hess, Lihua Y Marmorstein, Lonny R. Levin, Jochen Buck, Alan D Marmorstein

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Glaucoma is a leading cause of blindness affecting as many as 2.2 million Americans. All current glaucoma treatment strategies aim to reduce intraocular pressure (IOP). IOP results from the resistance to drainage of aqueous humor (AH) produced by the ciliary body in a process requiring bicarbonate. Once secreted into the anterior chamber, AH drains from the eye via two pathways: uveoscleral and pressure- dependent or conventional outflow(Ct). Modulation of "inflow" and "outflow" pathways is thought to occur via distinct, local mechanisms. Mice deficient in the bicarbonate channel bestrophin-2 (Best2), however, exhibit a lower IOP despite an increase inAHproduction. Best2 is expressed uniquely in nonpigmented ciliary epithelial (NPE) cells providing evidence for a bicarbonate- dependent communicative pathway linking inflow and outflow. Here, we show that bicarbonate-sensitive soluble adenylyl cyclase (sAC) is highly expressed in the ciliary body in NPE cells, but appears to be absent from drainage tissues. Pharmacologic inhibition of sAC in mice causes a significant increase in IOP due to a decrease in C t with no effect on inflow. In mice deficient in sAC IOP is elevated, and C t is decreased relative to wild-type mice. Pharmacologic inhibition of sAC did not alter IOP or C t in sAC deficient mice. Based on these data we propose that the ciliary body can regulate C t and that sAC serves as a critical sensor of bicarbonate in the ciliary body regulating the secretion of substances into the AH that govern outflow facility independent of pressure.

Original languageEnglish (US)
Pages (from-to)41353-41358
Number of pages6
JournalJournal of Biological Chemistry
Volume286
Issue number48
DOIs
StatePublished - Dec 2 2011
Externally publishedYes

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Ciliary Body
Anterior Chamber
Intraocular Pressure
Adenylyl Cyclases
Drainage
Bicarbonates
Aqueous Humor
Glaucoma
Epithelial Cells
Pressure
Blindness
Modulation
Tissue
Sensors

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Regulation of anterior chamber drainage by bicarbonatesensitive soluble adenylyl cyclase in the ciliary body. / Lee, Yong S.; Tresguerres, Martin; Hess, Kenneth; Marmorstein, Lihua Y; Levin, Lonny R.; Buck, Jochen; Marmorstein, Alan D.

In: Journal of Biological Chemistry, Vol. 286, No. 48, 02.12.2011, p. 41353-41358.

Research output: Contribution to journalArticle

Lee, Yong S. ; Tresguerres, Martin ; Hess, Kenneth ; Marmorstein, Lihua Y ; Levin, Lonny R. ; Buck, Jochen ; Marmorstein, Alan D. / Regulation of anterior chamber drainage by bicarbonatesensitive soluble adenylyl cyclase in the ciliary body. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 48. pp. 41353-41358.
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