TY - JOUR
T1 - Regulation of a novel immediate early response gene, IEX-1, in keratinocytes by 1α,25-dihydroxyvitamin D3
AU - Kobayashi, Teruaki
AU - Pittelkow, Mark R.
AU - Warner, Gina M.
AU - Squillace, Karen A.
AU - Kumar, Rajiv
N1 - Funding Information:
1 Address correspondence to: Dr. Mark Pittelkow, Mayo Clinic, 200 First, SW, Guggenheim 4, Rochester, MN 55905. Fax: 507-284-1086. E-mail: pittelkow.mark@mayo.edu or Dr. Rajiv Kumar, Mayo Clinic, 200 First St., SW, 911A Guggenheim Bldg., Rochester, MN 55905. Fax: 507-266-2710. E-mail: rkumar@mayo.edu. Supported by NIH Grant DK-25409 (RK), Dermatology Foundation Research Fellowship, 1997 (TK), and Mayo Foundation (RK, MRP).
PY - 1998/10/29
Y1 - 1998/10/29
N2 - 1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3] regulates cellular growth and differentiation. We show that in keratinocytes, 1α,25(OH)2D3 reduces concentrations of the messenger RNA of IEX-1, the product of which blocks Fas- or tumor necrosis factor type α-induced apoptosis in various cells. In sub-confluent keratinocyte cultures, the addition of 1α,25(OH)2D3 in amounts that induce growth arrest, reduces IEX-1 mRNA concentrations. In confluent cells, 1α,25(OH)2D3 initially reduces and then increases IEX-1 mRNA concentrations. IEX-1 protein is localized in the nucleus and perinuclear region of keratinocytes. In sub-confluent cells, 1α,25(OH)2D3 translocates IEX-1 protein from the nucleus to the perinuclear region and cytoplasm. Since IEX-1 has recently been shown to regulate cell survival and number, we suggest that IEX-1 may play a role in keratinocyte growth and differentiation and that 1α,25(OH)2D3 may reduce keratinocyte growth via a reduction in IEX-1 mRNA and a change in the intracellular distribution of IEX-1 protein.
AB - 1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3] regulates cellular growth and differentiation. We show that in keratinocytes, 1α,25(OH)2D3 reduces concentrations of the messenger RNA of IEX-1, the product of which blocks Fas- or tumor necrosis factor type α-induced apoptosis in various cells. In sub-confluent keratinocyte cultures, the addition of 1α,25(OH)2D3 in amounts that induce growth arrest, reduces IEX-1 mRNA concentrations. In confluent cells, 1α,25(OH)2D3 initially reduces and then increases IEX-1 mRNA concentrations. IEX-1 protein is localized in the nucleus and perinuclear region of keratinocytes. In sub-confluent cells, 1α,25(OH)2D3 translocates IEX-1 protein from the nucleus to the perinuclear region and cytoplasm. Since IEX-1 has recently been shown to regulate cell survival and number, we suggest that IEX-1 may play a role in keratinocyte growth and differentiation and that 1α,25(OH)2D3 may reduce keratinocyte growth via a reduction in IEX-1 mRNA and a change in the intracellular distribution of IEX-1 protein.
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U2 - 10.1006/bbrc.1998.9556
DO - 10.1006/bbrc.1998.9556
M3 - Article
C2 - 9791001
AN - SCOPUS:0032578848
SN - 0006-291X
VL - 251
SP - 868
EP - 873
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -