Regulation by glucocorticoids of interferon gamma-induced HLA-DR antigen expression in cultured human orbital fibroblasts

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Abstract

Objective - The purpose of this study was to determine whether glucocorticoids can block the induction of HLA-DR antigen expression by interferon gamma in human fibroblasts in culture. Design and patients - Confluent cultures of fibroblasts derived from the orbit or the skin of patients with Graves' ophthalmopathy or from normal subjects were treated with interferon gamma (100 U/ml) without or with graded concentrations of steroids. Measurements - Cultures were analysed for HLA-DR expression using quantitative immunoblotting and indirect immunofluorescence. Results - Glucocorticoids could block HLA-DR induction in a dose-dependent manner. At 10-6 mol/l, the steroids dexamethasone and RU 28362 inhibited expression by 70% (P < 0.004) and 56% (P < 0.002) respectively. RU 38486, a glucocorticoid antagonist, could reverse the effect of both glucocorticoids, an action that was also dose dependent. At 10-5 mol/l, RU 38486 blocked virtually the entire glucocorticoid effect. Conclusions - These results suggest that glucocorticoids can regulate HLA-DR induction by interferon gamma at physiological concentrations, an action which is stereo-specific and mediated through the glucocorticoid receptor.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalClinical Endocrinology
Volume37
Issue number1
StatePublished - 1992

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ASJC Scopus subject areas

  • Endocrinology

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