Regional T1 relaxation time constants in Ex vivo human brain: Longitudinal effects of formalin exposure

Mekala R. Raman, Yunhong Shu, Timothy G. Lesnick, Clifford R Jr. Jack, Kejal M Kantarci

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Relaxation time constants are useful as markers of tissue properties. Imaging ex vivo tissue is done for research purposes; however, T1 relaxation time constants are altered by tissue fixation in a time-dependent manner. This study investigates regional changes in T1 relaxation time constants in ex vivo brain tissue over 6 months of fixation. Methods: Five ex vivo human brain hemispheres in 10% formalin were scanned over 6 months. Mean T1 relaxation time constants were measured in regions of interest (ROIs) representing gray matter (GM) and white matter (WM) regions and analyzed as a function of fixation time. Results: Cortical GM ROIs had longer T1 relaxation time constants than WM ROIs; the thalamus had T1 relaxation time constants similar to those of WM ROIs. T1 relaxation time constants showed rapid shortening within the first 6 weeks after fixation followed by a slower rate of decline. Conclusion: Both GM and WM T1 relaxation time constants of fixed brain tissue show rapid decline within the first 6 weeks after autopsy and slow by 6 months. This information is useful for optimizing MR imaging acquisition parameters according to fixation time for ex vivo brain imaging studies.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - 2016

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Formaldehyde
Brain
Tissue Fixation
Thalamus
Neuroimaging
Autopsy
White Matter

Keywords

  • Brain
  • Ex vivo
  • Fixation
  • MRI
  • Neuroimaging
  • T1-mapping

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Regional T1 relaxation time constants in Ex vivo human brain: Longitudinal effects of formalin exposure",
abstract = "Purpose: Relaxation time constants are useful as markers of tissue properties. Imaging ex vivo tissue is done for research purposes; however, T1 relaxation time constants are altered by tissue fixation in a time-dependent manner. This study investigates regional changes in T1 relaxation time constants in ex vivo brain tissue over 6 months of fixation. Methods: Five ex vivo human brain hemispheres in 10{\%} formalin were scanned over 6 months. Mean T1 relaxation time constants were measured in regions of interest (ROIs) representing gray matter (GM) and white matter (WM) regions and analyzed as a function of fixation time. Results: Cortical GM ROIs had longer T1 relaxation time constants than WM ROIs; the thalamus had T1 relaxation time constants similar to those of WM ROIs. T1 relaxation time constants showed rapid shortening within the first 6 weeks after fixation followed by a slower rate of decline. Conclusion: Both GM and WM T1 relaxation time constants of fixed brain tissue show rapid decline within the first 6 weeks after autopsy and slow by 6 months. This information is useful for optimizing MR imaging acquisition parameters according to fixation time for ex vivo brain imaging studies.",
keywords = "Brain, Ex vivo, Fixation, MRI, Neuroimaging, T1-mapping",
author = "Raman, {Mekala R.} and Yunhong Shu and Lesnick, {Timothy G.} and Jack, {Clifford R Jr.} and Kantarci, {Kejal M}",
year = "2016",
doi = "10.1002/mrm.26140",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Regional T1 relaxation time constants in Ex vivo human brain

T2 - Longitudinal effects of formalin exposure

AU - Raman, Mekala R.

AU - Shu, Yunhong

AU - Lesnick, Timothy G.

AU - Jack, Clifford R Jr.

AU - Kantarci, Kejal M

PY - 2016

Y1 - 2016

N2 - Purpose: Relaxation time constants are useful as markers of tissue properties. Imaging ex vivo tissue is done for research purposes; however, T1 relaxation time constants are altered by tissue fixation in a time-dependent manner. This study investigates regional changes in T1 relaxation time constants in ex vivo brain tissue over 6 months of fixation. Methods: Five ex vivo human brain hemispheres in 10% formalin were scanned over 6 months. Mean T1 relaxation time constants were measured in regions of interest (ROIs) representing gray matter (GM) and white matter (WM) regions and analyzed as a function of fixation time. Results: Cortical GM ROIs had longer T1 relaxation time constants than WM ROIs; the thalamus had T1 relaxation time constants similar to those of WM ROIs. T1 relaxation time constants showed rapid shortening within the first 6 weeks after fixation followed by a slower rate of decline. Conclusion: Both GM and WM T1 relaxation time constants of fixed brain tissue show rapid decline within the first 6 weeks after autopsy and slow by 6 months. This information is useful for optimizing MR imaging acquisition parameters according to fixation time for ex vivo brain imaging studies.

AB - Purpose: Relaxation time constants are useful as markers of tissue properties. Imaging ex vivo tissue is done for research purposes; however, T1 relaxation time constants are altered by tissue fixation in a time-dependent manner. This study investigates regional changes in T1 relaxation time constants in ex vivo brain tissue over 6 months of fixation. Methods: Five ex vivo human brain hemispheres in 10% formalin were scanned over 6 months. Mean T1 relaxation time constants were measured in regions of interest (ROIs) representing gray matter (GM) and white matter (WM) regions and analyzed as a function of fixation time. Results: Cortical GM ROIs had longer T1 relaxation time constants than WM ROIs; the thalamus had T1 relaxation time constants similar to those of WM ROIs. T1 relaxation time constants showed rapid shortening within the first 6 weeks after fixation followed by a slower rate of decline. Conclusion: Both GM and WM T1 relaxation time constants of fixed brain tissue show rapid decline within the first 6 weeks after autopsy and slow by 6 months. This information is useful for optimizing MR imaging acquisition parameters according to fixation time for ex vivo brain imaging studies.

KW - Brain

KW - Ex vivo

KW - Fixation

KW - MRI

KW - Neuroimaging

KW - T1-mapping

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