Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion

Jasna Fejzo, Andrzej M. Krezel, William M. Westler, Slobodan I Macura, John L. Markley

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effect of internal motion on the quality of a protein structure derived from nuclear magnetic resonance (NMR) cross relaxation has been investigated experimentally. Internal rotation of the tyrosine-31 ring of turkey ovomucoid third domain was found to mediate magnetization transfer; the effect led to underestimation of proton-proton distances in its immediate neighborhood. Experimental methods that distinguish pure cross relaxation from chemical exchange mediated cross relaxation were used to separate true distances from distorted ones. Uncorrected and corrected sets of distances, where the corrections took internal motion into account, each were used as input to a distance geometry program for structural modeling. Each set of distances yielded a family of similar (converged) structures. The two families of structures differed considerably (2 Å) in the region of tyrosine-31. In addition, differences as large as 1 Å were observed at other positions throughout the structure. These results emphasize the importance of analyzing the effects of internal motions in order to obtain more accurate NMR solution structures.

Original languageEnglish (US)
Pages (from-to)3807-3811
Number of pages5
JournalBiochemistry
Volume30
Issue number16
StatePublished - 1991

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Tyrosine
Protons
Magnetic Resonance Spectroscopy
Ovomucin
Nuclear magnetic resonance
Magnetization
Proteins
Geometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion. / Fejzo, Jasna; Krezel, Andrzej M.; Westler, William M.; Macura, Slobodan I; Markley, John L.

In: Biochemistry, Vol. 30, No. 16, 1991, p. 3807-3811.

Research output: Contribution to journalArticle

Fejzo, Jasna ; Krezel, Andrzej M. ; Westler, William M. ; Macura, Slobodan I ; Markley, John L. / Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion. In: Biochemistry. 1991 ; Vol. 30, No. 16. pp. 3807-3811.
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