Reengineering paramyxovirus tropism

Elizabeth M. Hadac, Kah-Whye Peng, Takafumi Nakamura, Stephen J Russell

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Receptor specificity is a critical determinant of viral tropism, but the capacity of viruses to switch to alternative receptors has not been extensively studied. Here, we engineered the attachment protein of an attenuated measles virus and generated truly retargeted viruses that are blind to the native receptors CD46 and SLAM, but which propagate efficiently and exclusively via alternative cellular receptors, epidermal growth factor receptor, or CD38. The engineered receptor tropisms were stably maintained during multiple serial virus passage without reversion to native receptor usage, even on cells offering the choice of both native and alternative receptors. We conclude that paramyxoviruses have a remarkably flexible and adaptable entry mechanism.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalVirology
Volume329
Issue number2
DOIs
StatePublished - Nov 24 2004

Fingerprint

Tropism
Viruses
Viral Tropism
Serial Passage
Measles virus
Epidermal Growth Factor Receptor
Proteins

Keywords

  • Paramyxovirus
  • Receptor specificity
  • Tropism

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Reengineering paramyxovirus tropism. / Hadac, Elizabeth M.; Peng, Kah-Whye; Nakamura, Takafumi; Russell, Stephen J.

In: Virology, Vol. 329, No. 2, 24.11.2004, p. 217-225.

Research output: Contribution to journalArticle

Hadac, Elizabeth M. ; Peng, Kah-Whye ; Nakamura, Takafumi ; Russell, Stephen J. / Reengineering paramyxovirus tropism. In: Virology. 2004 ; Vol. 329, No. 2. pp. 217-225.
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