Reducing intra-sample variability of muscle lipid analysis

Lianzhen Zhou, ZengKui Guo

Research output: Contribution to journalArticle

Abstract

Intra-sample variability of intramyocellular triacylglycerols (imcTG) in skeletal muscle has been examined using two different tissue processing sequences assisted by tracer and solvent extraction techniques. Aliquoting after homogenization of muscle biopsies significantly reduced intra-sample variability compared to the aliquoting before homogenization approach. The results suggested that skeletal muscle samples be homogenized before aliquoting, if necessary, in order to reduce variability in imcTG measurements. Intramyocellular non-esterified fatty acids have a lower variability than triacylglycerols.

Original languageEnglish (US)
Pages (from-to)1047-1050
Number of pages4
JournalEuropean Journal of Lipid Science and Technology
Volume108
Issue number12
DOIs
StatePublished - 2006

Fingerprint

Lipids
Muscle
Triglycerides
Muscles
muscles
triacylglycerols
Skeletal Muscle
lipids
homogenization
skeletal muscle
Biopsy
Solvent extraction
Fatty acids
sampling
Fatty Acids
Tissue
free fatty acids
tracer techniques
biopsy
Processing

Keywords

  • Lipids
  • Skeletal muscle
  • Variability

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Reducing intra-sample variability of muscle lipid analysis. / Zhou, Lianzhen; Guo, ZengKui.

In: European Journal of Lipid Science and Technology, Vol. 108, No. 12, 2006, p. 1047-1050.

Research output: Contribution to journalArticle

Zhou, Lianzhen ; Guo, ZengKui. / Reducing intra-sample variability of muscle lipid analysis. In: European Journal of Lipid Science and Technology. 2006 ; Vol. 108, No. 12. pp. 1047-1050.
@article{b263fe5238174a3abc1f4801caa79e71,
title = "Reducing intra-sample variability of muscle lipid analysis",
abstract = "Intra-sample variability of intramyocellular triacylglycerols (imcTG) in skeletal muscle has been examined using two different tissue processing sequences assisted by tracer and solvent extraction techniques. Aliquoting after homogenization of muscle biopsies significantly reduced intra-sample variability compared to the aliquoting before homogenization approach. The results suggested that skeletal muscle samples be homogenized before aliquoting, if necessary, in order to reduce variability in imcTG measurements. Intramyocellular non-esterified fatty acids have a lower variability than triacylglycerols.",
keywords = "Lipids, Skeletal muscle, Variability",
author = "Lianzhen Zhou and ZengKui Guo",
year = "2006",
doi = "10.1002/ejlt.200600171",
language = "English (US)",
volume = "108",
pages = "1047--1050",
journal = "European Journal of Lipid Science and Technology",
issn = "1438-7697",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - Reducing intra-sample variability of muscle lipid analysis

AU - Zhou, Lianzhen

AU - Guo, ZengKui

PY - 2006

Y1 - 2006

N2 - Intra-sample variability of intramyocellular triacylglycerols (imcTG) in skeletal muscle has been examined using two different tissue processing sequences assisted by tracer and solvent extraction techniques. Aliquoting after homogenization of muscle biopsies significantly reduced intra-sample variability compared to the aliquoting before homogenization approach. The results suggested that skeletal muscle samples be homogenized before aliquoting, if necessary, in order to reduce variability in imcTG measurements. Intramyocellular non-esterified fatty acids have a lower variability than triacylglycerols.

AB - Intra-sample variability of intramyocellular triacylglycerols (imcTG) in skeletal muscle has been examined using two different tissue processing sequences assisted by tracer and solvent extraction techniques. Aliquoting after homogenization of muscle biopsies significantly reduced intra-sample variability compared to the aliquoting before homogenization approach. The results suggested that skeletal muscle samples be homogenized before aliquoting, if necessary, in order to reduce variability in imcTG measurements. Intramyocellular non-esterified fatty acids have a lower variability than triacylglycerols.

KW - Lipids

KW - Skeletal muscle

KW - Variability

UR - http://www.scopus.com/inward/record.url?scp=33846152576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846152576&partnerID=8YFLogxK

U2 - 10.1002/ejlt.200600171

DO - 10.1002/ejlt.200600171

M3 - Article

AN - SCOPUS:33846152576

VL - 108

SP - 1047

EP - 1050

JO - European Journal of Lipid Science and Technology

JF - European Journal of Lipid Science and Technology

SN - 1438-7697

IS - 12

ER -