Reducing intra-sample variability of muscle lipid analysis

Lianzhen Zhou, Zeng Kui Guo

Research output: Contribution to journalArticle

Abstract

Intra-sample variability of intramyocellular triacylglycerols (imcTG) in skeletal muscle has been examined using two different tissue processing sequences assisted by tracer and solvent extraction techniques. Aliquoting after homogenization of muscle biopsies significantly reduced intra-sample variability compared to the aliquoting before homogenization approach. The results suggested that skeletal muscle samples be homogenized before aliquoting, if necessary, in order to reduce variability in imcTG measurements. Intramyocellular non-esterified fatty acids have a lower variability than triacylglycerols.

Original languageEnglish (US)
Pages (from-to)1047-1050
Number of pages4
JournalEuropean Journal of Lipid Science and Technology
Volume108
Issue number12
DOIs
StatePublished - Dec 1 2006

Keywords

  • Lipids
  • Skeletal muscle
  • Variability

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Chemistry(all)
  • Industrial and Manufacturing Engineering

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